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Preparation of Silica Nanoparticles Through Microwave-assisted Acid-catalysis
Published on: December 16, 2013
A quick accelerating microwave-assisted sustainable technique: permutated spiro-casing for imaging experiment
Animesh Mondal1, Barnali Naskar1, Sanchita Goswami1
1Department of Chemistry, University of Calcutta, 92 APC Road, Kolkata, 700009, India.
Abstract:
A quick access tool for the one-pot, chromatography-free synthesis of the diversified dihydrospiro[indeno[1,2-b]pyridine-4,3'-indoline or acenaphthylene-1,4'-indeno[1,2-b]pyridine spiro-analogous via sustainable microwave condition in minimal 1:1 (v/v) aqueous ethanol without any metal catalyst is demonstrated here. This permutated spiro-casing was designed as fluorescence probe at physiological pH for selective detection of Zn2+, even in the presence of other competitive ions and showed a fluorescent enhancement with 1:1 metal/ligand complex. Moreover, this spiro sensor was successfully applied as an effective intracellular Zn2+ imaging agent in the biomedical study of human hepatocellular liver carcinoma cells (HepG2) due to its cell permeability property. A quick access technique for the permutated dihydrospiro-pyridine via chromatography-free sustainable microwave condition and its applications as organic fluorescence probe at physiological pH for selective detection of Zn2+ and effective intracellular Zn2+ imaging in HepG2 cells.
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