Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Urinary endo-beta-galactosidase capable of depolymerizing polylactosaminoglycans.

R DeGasperi, Y T Li, S C Li

    The Journal of Biological Chemistry
    |May 5, 1986
    PubMed
    Summary

    Human urine contains a novel endo-beta-galactosidase enzyme that breaks down polylactosaminoglycans. This discovery reveals a new metabolic pathway for these complex carbohydrates.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Combining EBV DNA dynamics with RECIST-based radiographic response to improve prognostic stratification of recurrent/metastatic nasopharyngeal carcinoma in the era of immunotherapy.

    Rhinology·2026
    Same author

    [Recurrent myocardial infarction caused by left atrial appendage thrombus diagnosed by transesophageal echocardiography: a case report].

    Zhonghua xin xue guan bing za zhi·2026
    Same author

    [Mechanism of Qizao oral liquid in the treatment of lead poisoning based on network pharmacology and molecular docking technology].

    Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2025
    Same author

    Measurement of Electron Antineutrino Oscillation Amplitude and Frequency via Neutron Capture on Hydrogen at Daya Bay.

    Physical review letters·2024
    Same author

    Search for a Sub-eV Sterile Neutrino Using Daya Bay's Full Dataset.

    Physical review letters·2024
    Same author

    Improved Measurement of the Evolution of the Reactor Antineutrino Flux and Spectrum at Daya Bay.

    Physical review letters·2023

    Area of Science:

    • Biochemistry
    • Glycobiology
    • Enzymology

    Background:

    • Polylactosaminoglycans are complex carbohydrates found in various tissues.
    • The degradation pathways of these glycans are not fully understood.

    Purpose of the Study:

    • To investigate the presence and activity of endo-beta-galactosidase in human urine.
    • To characterize the enzyme's ability to depolymerize sulfated and non-sulfated polylactosaminoglycans.

    Main Methods:

    • Enzyme purification using DEAE-Sephadex A-50 and concanavalin A-Sepharose chromatography.
    • Analysis of enzymatic products from keratan sulfates and erythroglycan using chromatographic methods.

    Main Results:

    • Human urine contains an endo-beta-galactosidase that depolymerizes polylactosaminoglycans.
    • The enzyme liberates specific disaccharides and oligosaccharides from various sources, including keratan sulfates and erythroglycan.
    • The resulting oligosaccharide profiles are similar to those produced by Escherichia freundii endo-beta-galactosidase.

    Conclusions:

    • The presence of urinary endo-beta-galactosidase suggests a novel catabolic pathway for polylactosaminoglycans.
    • This pathway involves the cleavage of internal beta-galactosyl linkages within the glycan chain.

    Related Experiment Videos