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Interleukin 1 production by human polymorphonuclear neutrophils.
Journal of Immunology (Baltimore, Md. : 1950)
|May 15, 1986
Summary
Human polymorphonuclear neutrophils (PMN) can produce Interleukin-1 (IL-1), a factor that stimulates thymocyte proliferation. This study confirms PMN as a source of IL-1, demonstrating its biological activity and characteristics.
Area of Science:
- Immunology
- Cell Biology
Background:
- Human polymorphonuclear neutrophils (PMN) share lineage and characteristics with macrophages.
- Interleukin-1 (IL-1) resembles endogenous pyrogen (EP), and PMN have been controversially cited as a potential source of EP.
Purpose of the Study:
- To determine if human PMN can produce IL-1.
- To characterize the properties and functions of PMN-derived IL-1.
Main Methods:
- Stimulation of human PMN with zymosan and phorbol myristic acetate.
- Assays for thymocyte proliferation and synovial fibroblast proliferation.
- Biochemical characterization including heat lability, pH stability, and gel filtration.
Main Results:
- PMN produced a factor (PMN IL-1) that induced mouse thymocyte proliferation.
- PMN IL-1 release was dose- and time-dependent.
- PMN IL-1 was heat-labile, pH-sensitive, and had an estimated molecular weight of 13,000-17,000.
- PMN IL-1 stimulated synovial fibroblast proliferation and plasminogen activator release.
Conclusions:
- Human PMN can be stimulated to produce IL-1.
- PMN-derived IL-1 exhibits biological activities similar to macrophage-derived IL-1.
- This finding supports PMN as a significant source of IL-1 in inflammatory responses.