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Updated: Jan 28, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
Published on: September 4, 2019
Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library
Xi Qin1, Wenrong Yao1, Xinchang Shi1
1Department of Recombinant Products, National Institutes for Food and Drug Control, Beijing, 100050, China.
Abstract:
Bioassay of recombinant protein products is important tests to ensure protein effectiveness. Some recombinant protein products have no cells used in their bioassay but instead use animal models, while others have no suitable method. Here, we developed a method to obtain responsive cells used in bioassay of proteins. After screening of a CRISPR/Cas9 library, we obtained a responsive cell line that grew faster in the presence of rhEGF (recombinant human epidermal growth factor) than that of control cells. We used this cell line for bioassay of rhEGF. This cell line, compared with the control cells, had a 2 day shorter operation time and had lower interference. The responsive cell line is more suitable for use in bioassay of rhEGF.
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