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Determination of melphalan in human plasma by UPLC-UV method
Liusheng Huang1, Vincent Cheah2, Danna Chan3
1Drug Research Unit, Department of Clinical Pharmacy, School of Pharmacy, University of California at San Francisco, San Francisco, CA, 94110, USA. liusheng.huang@ucsf.edu.
Cancer Chemotherapy and Pharmacology
|March 9, 2019
Summary
A new UPLC-PDA method rapidly quantifies unstable melphalan (MPL) in human plasma. This validated assay enables accurate drug monitoring in clinical settings, including pediatric pharmacokinetic studies.
Area of Science:
- Analytical Chemistry
- Pharmacokinetics
Background:
- Melphalan (MPL) is an alkylating agent used in chemotherapy.
- MPL is chemically unstable, necessitating rapid and accurate quantification methods.
- Accurate drug monitoring is crucial for optimizing therapeutic efficacy and minimizing toxicity.
Purpose of the Study:
- To develop and validate a fast Ultra-Performance Liquid Chromatography-Photodiode Array (UPLC-PDA) method for quantifying melphalan (MPL) in human plasma.
- To establish a reliable analytical tool for therapeutic drug monitoring of MPL.
Main Methods:
- Plasma samples were prepared by protein precipitation using acetylmelphalan as an internal standard and trichloroacetic acid.
- Melphalan was separated on an Acquity™ BEH C18 LC column using a gradient elution with ammonium acetate and acetonitrile.
- Quantification was performed using a UPLC-PDA system with detection at 261 nm, achieving a total run time of 4 minutes.
Main Results:
- The developed UPLC-PDA method provided a rapid quantification of melphalan with a total run time of 4 minutes per sample.
- The calibration range was established from 100-40,000 ng/mL, with a lower limit of quantification of 100 ng/mL.
- The method demonstrated good performance and was validated according to FDA guidelines.
Conclusions:
- The UPLC-PDA method is suitable for the rapid and accurate quantification of melphalan in human plasma.
- This validated assay can be effectively applied to clinical pharmacokinetic studies, including those in pediatric populations.
- The method facilitates timely therapeutic drug monitoring for melphalan.
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