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TRIM27 Promotes Hepatitis C Virus Replication by Suppressing Type I Interferon Response.

Feng Zheng1, Nannan Xu2, Yajun Zhang2

  • 1Department of Infectious Disease, Qilu Hospital of Shandong University, 107# West Wenhua Road, Jinan, 250012, Shandong province, People's Republic of China. zhengfqlh@163.com.

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Tripartite motif 27 (TRIM27) enhances hepatitis C virus (HCV) replication by suppressing the type I interferon immune response. Targeting TRIM27 could be a new strategy for controlling HCV infection.

Keywords:
IRF3NF-κBTRIM27hepatitis C virustype I interferon response

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Area of Science:

  • Virology
  • Immunology
  • Hepatology

Background:

  • Type I interferon (IFN) response is crucial for antiviral defense.
  • The role of Tripartite motif 27 (TRIM27) in hepatitis C virus (HCV) replication is unknown.
  • TRIM27 is involved in antiviral innate immunity.

Purpose of the Study:

  • To investigate the effect of TRIM27 on HCV replication.
  • To elucidate the underlying mechanisms of TRIM27's action in HCV infection.

Main Methods:

  • Assessing TRIM27 expression in HCV-infected cells and IFN-stimulated cells.
  • Evaluating the impact of TRIM27 overexpression and knockdown on viral RNA and protein levels.
  • Analyzing the effect of TRIM27 mutants on IRF3 and NF-κB pathways and HCV replication.

Main Results:

  • TRIM27 expression is upregulated during HCV infection and IFN stimulation.
  • TRIM27 positively regulates HCV replication, as shown by increased viral RNA and protein with overexpression and decreased levels with knockdown.
  • TRIM27 inhibits the type I IFN response by suppressing the IRF3 and NF-κB pathways, which is essential for its role in promoting HCV replication.

Conclusions:

  • TRIM27 is identified as a novel positive regulator of HCV replication.
  • TRIM27 promotes HCV replication by inhibiting the host's type I IFN antiviral response.
  • Targeting TRIM27 presents a potential therapeutic strategy for managing HCV infection.