Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cis-regulatory Sequences

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

Protein and Protein Structure

Protein and Protein Structure

Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...

Conservation of Protein Domains Over Different Proteins

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain. Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Protein Networks

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

Protein Families

Protein Families

Protein families are groups of homologous proteins; that is, they have similarities in amino acid sequences and three-dimensional structures. Protein families usually occur because of gene duplication, where an additional copy of a gene is inserted into the genome of an organism. Mutations that change the amino acids but still allow the protein to be properly synthesized, will lead to new protein family members. If these new proteins contain similar amino acids in key...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Virome-wide ubiquitin ligase discovery reveals diverse mechanisms of immune evasion.

Science (New York, N.Y.)·2026

Same author

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026

Same author

Persistence of memory: lifespan dynamics of the human antiviral antibody reactome.

Nature communications·2026

Same author

Systems-Scale Structural Modeling Reveals the Germline Architecture of Immunodominance.

bioRxiv : the preprint server for biology·2026

Same author

Integrative Molecular Analyses of Inflammatory and Autoimmune Signals in Cardiac Sarcoidosis.

Circulation·2026

Same author

A glycan-based adjuvant expands the breadth and duration of protection of mRNA-based vaccines.

Nature immunology·2026

Search research articles

Related Experiment Video

Updated: Jan 28, 2026

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Sequencer Hacking Unlocks Quantitative Protein Studies.

Karl W Barber¹, Stephen J Elledge¹

¹Division of Genetics, Brigham and Women's Hospital, Howard Hughes Medical Institute; Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.

|March 9, 2019

Summary

Researchers repurposed DNA sequencers for protein studies. This method identifies better protein variants and analyzes amino acid effects on enzyme activity, advancing biochemical research.

More Related Videos

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Published on: July 16, 2017

Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo

Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo

Published on: June 28, 2019

Related Experiment Videos

Last Updated: Jan 28, 2026

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Published on: July 16, 2017

Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo

Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo

Published on: June 28, 2019

Area of Science:

Biochemistry
Molecular Biology
Genomics

Background:

High-throughput screening is crucial for understanding protein function.
Next-generation sequencing (NGS) technologies are primarily used for DNA analysis.

Purpose of the Study:

To adapt NGS platforms for high-throughput protein biochemical studies.
To identify sequence variants that enhance protein-protein interactions.
To dissect the role of specific amino acids in enzymatic activity.

Main Methods:

Repurposing a common next-generation DNA sequencer.
Developing novel assays for protein biochemical analysis using sequencing data.
Analyzing sequence variants to correlate with protein function.

Main Results:

Successfully adapted NGS for protein studies.
Identified sequence variants leading to stronger protein-protein interactions.
Dissected the contributions of individual amino acids to enzymatic activity.

Conclusions:

NGS platforms can be repurposed for high-throughput protein biochemistry.
This approach facilitates the discovery of improved protein variants.
Provides insights into structure-function relationships of proteins.