Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Global Positioning System01:30

Introduction to Global Positioning System

505
The Global Positioning System (GPS) revolutionized positioning on Earth, providing precise location data through satellite ranging. The GPS system was developed in 1978 by the U.S. Department of Defense  for military use, and it became available for civilian applications in 1983, transforming fields including navigation, fleet management, and time synchronization for telecommunications systems.GPS consists of satellites in medium Earth orbit, about 20,200 kilometers above the surface,...
505
Errors in Global Positioning System01:26

Errors in Global Positioning System

347
Global Positioning System (GPS) technology has revolutionized navigation and positioning, but its accuracy is often compromised by various errors. These errors, stemming from environmental, satellite, and receiver-related factors, require careful mitigation to ensure reliable performance across applications.Atmospheric ErrorsGPS signals travel through the Earth’s ionosphere and troposphere, introducing delays which affect accuracy. The ionosphere is strongly influenced by charged particles,...
347
Field Application of Global Positioning System01:28

Field Application of Global Positioning System

325
The Global Positioning System (GPS) has become an indispensable tool in fieldwork, offering unparalleled precision and efficiency for surveying, navigation, and infrastructure development. By harnessing signals from a constellation of satellites, GPS receivers determine the location of objects with remarkable speed and accuracy, often completing calculations within a second.Advantages of Modern GPS TechnologyContemporary GPS receivers are designed to meet the practical demands of field...
325
Types of Global Positioning System Surveys01:30

Types of Global Positioning System Surveys

356
GPS surveying methods vary in application, accuracy, and data collection techniques, catering to diverse surveying and mapping needs. Static GPS, kinematic GPS, and real-time kinematic (RTK) surveying are widely used. Each technique offers distinct advantages.Static GPS involves placing one receiver at a known reference point and another at the target point. It collects exact positional data by observing multiple satellite ranges over an extended period, achieving centimeter-level accuracy for...
356
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

9.9K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.9K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

18.6K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional CRISPR screens reveal TPL1 lncRNA as a regulator of triple-negative breast cancer hallmarks.

Journal of advanced research·2026
Same author

Translon: a single term for translated regions.

Nature methods·2025
Same author

Evolutionarily recent transcription factors partake in human cell cycle regulation.

Cell genomics·2025
Same author

Targeting Wnt signalling through LINC02418: insights from CRISPR screens.

Gut·2025
Same author

GENCODE 2025: reference gene annotation for human and mouse.

Nucleic acids research·2024
Same author

GENCODE: massively expanding the lncRNA catalog through capture long-read RNA sequencing.

bioRxiv : the preprint server for biology·2024

Related Experiment Video

Updated: Jan 28, 2026

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

9.3K

Global Positioning System: Understanding Long Noncoding RNAs through Subcellular Localization.

Joana Carlevaro-Fita1, Rory Johnson1

  • 1Department of Medical Oncology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland; Department for BioMedical Research, University of Bern, Bern, Switzerland.

Molecular Cell
|March 9, 2019
PubMed
Summary
This summary is machine-generated.

The location of long noncoding RNAs (lncRNAs) in the cell dictates their function. New analysis frameworks reveal abundant cytoplasmic lncRNAs with diverse roles, challenging previous nuclear-centric views.

More Related Videos

Isolation of Small Noncoding RNAs from Human Serum
06:44

Isolation of Small Noncoding RNAs from Human Serum

Published on: June 19, 2014

18.6K
Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
09:59

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Published on: March 16, 2017

9.4K

Related Experiment Videos

Last Updated: Jan 28, 2026

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

9.3K
Isolation of Small Noncoding RNAs from Human Serum
06:44

Isolation of Small Noncoding RNAs from Human Serum

Published on: June 19, 2014

18.6K
Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
09:59

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Published on: March 16, 2017

9.4K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their diverse biological roles.
  • Traditionally, lncRNAs were primarily viewed as nuclear-restricted regulators of gene transcription and chromatin structure.
  • Emerging evidence highlights a significant population of lncRNAs residing in the cytoplasm, performing functions beyond transcriptional regulation.

Purpose of the Study:

  • To propose a new analytical framework for sequencing data to better understand lncRNA localization.
  • To investigate the functional implications of lncRNA subcellular localization.
  • To highlight the potential for cytoplasmic lncRNAs to rival nuclear populations in abundance and functional significance.

Main Methods:

  • Development of a novel computational framework for analyzing sequencing-based data.
  • Review and integration of existing high-resolution RNA mapping data.
  • Consideration of emerging techniques for transcriptome-wide organelle-associated RNA mapping.

Main Results:

  • The proposed framework suggests that cytoplasmic lncRNA molecule numbers can be comparable to nuclear lncRNA populations.
  • High-resolution RNA mapping reveals specific localization patterns for lncRNAs within the cell.
  • Evidence points towards sequence-based mechanisms guiding lncRNA localization and function.

Conclusions:

  • Subcellular localization is a critical determinant of lncRNA molecular functions.
  • The discovery of abundant cytoplasmic lncRNAs expands our understanding of their roles beyond the nucleus.
  • Further high-resolution mapping will be essential to fully elucidate the functions of lncRNAs in various cellular compartments.