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Updated: Jan 28, 2026

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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CRISPR/Cas9: a powerful tool for identification of new targets for cancer treatment

Bin Liu1, Ali Saber1, Hidde J Haisma1

  • 1Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, The Netherlands.

Drug Discovery Today
|March 9, 2019
PubMed

Insights

The CRISPR/Cas9 genome-editing tool revolutionizes cancer research by identifying novel therapeutic targets. This technology effectively distinguishes between mutant and wild-type alleles, paving the way for new cancer treatments.

Area of Science:

  • Biotechnology
  • Genetics
  • Oncology

Background:

  • The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated nuclease 9 (Cas9) system is a revolutionary genome-editing tool.
  • CRISPR/Cas9 is extensively utilized to explore the molecular underpinnings of various cancer types.

Purpose of the Study:

  • To review recent advancements in CRISPR/Cas9 applications for identifying potential molecular targets in cancer.
  • To highlight the efficacy of CRISPR/Cas9 in differentiating between mutant and wild-type alleles in cancer contexts.

Main Methods:

  • Literature review of studies employing CRISPR/Cas9 technology in cancer research.
  • Analysis of identified therapeutic targets and noncoding RNAs (ncRNAs) discovered through CRISPR/Cas9 applications.

Main Results:

  • CRISPR/Cas9 has successfully identified several promising therapeutic targets, including CD38, CXCR2, MASTL, and RBX2.
  • The technology has also led to the discovery of novel noncoding RNAs (ncRNAs) with potential roles in cancer.
  • CRISPR/Cas9 demonstrates effectiveness in distinguishing between mutant and wild-type alleles, crucial for targeted therapies.

Conclusions:

  • CRISPR/Cas9 is a powerful tool for advancing cancer research and identifying therapeutic targets.
  • The technology holds significant promise for developing novel cancer treatments by enabling precise genetic manipulation and target identification.
  • Challenges and obstacles related to the therapeutic application of CRISPR/Cas9 warrant further investigation.

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