Uptake and toxicity studies of magnetic TiO₂-Based nanophotocatalyst in Arabidopsis thaliana

Information on the environmental impact of magnetic TiO₂-based nanophotocatalysts is scarce. This study evaluated the potential effects of an innovative magnetic nanophotocatalyst N-TiO₂/Fe₃O₄@SiO₂ (NTFS) on plants using Arabidopsis thaliana grown in a hydroponic system. NTFS was detected in the vascular tissues and mesophyll of plants, thus confirming the uptake and upwards transport of NTFS from roots to leaves. Fourier transform infrared spectroscopy was applied to determine compositional and structural alterations in plant tissues exposed to NTFS, or its two main components (N-TiO₂ and Fe₃O₄@SiO₂), at concentrations ranging from 0 to 1000 mg/L, but no changes were detected in the lipids, pectins, proteins, cellulose, hemicellulose, and carbohydrates. The morphology and biomass of the plants were not affected by the NTFS or its components either. Biosensors for inorganic phosphate (Pi) and MgATP^2- were used to monitor the in vivo Pi and MgATP^2- levels in the plant cells. The results showed that NTFS and its components did not induce any adverse effects on the cytosolic Pi level or ATP synthesis, indicating the energy physiology of Arabidopsis was unaffected. In general, NTFS has inconsequential toxic effects on Arabidopsis, but can be taken up by plants, enter the food chain, and cause potential exposure and bioaccumulation in animals and human beings.