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Updated: Jan 28, 2026

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation
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Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Published on: May 31, 2016

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Bone marrow mesenchymal stem cell-derived exosomes alleviate high phosphorus-induced vascular smooth muscle cells

Yan Guo1, Shumin Bao1, Wang Guo1

  • 1Department of Nephrology, Beijing Friendship Hospital, Faculty of Kidney Diseases, Capital Medical University, Beijing, 100050, China.

Functional & Integrative Genomics
|March 10, 2019
PubMed

Insights

Bone marrow mesenchymal stem cell-derived exosomes (EXO) reduce vascular calcification in chronic kidney disease (CKD) patients. EXO therapy improves calcification by altering microRNA profiles, offering a potential therapeutic strategy.

Area of Science:

  • Biomedical research
  • Cell biology
  • Vascular biology

Background:

  • Vascular calcification is a significant complication in chronic kidney disease (CKD), predicting cardiovascular disease and mortality.
  • Bone marrow mesenchymal stem cell (BMSC) therapy shows promise in reducing vascular calcification, but mechanisms require elucidation.

Purpose of the Study:

  • To investigate the mechanisms by which BMSC-derived exosomes (EXO) ameliorate vascular calcification.
  • To analyze the impact of EXO on human aortic vascular smooth muscle cells (HA-VSMCs) under calcifying conditions.

Main Methods:

  • Isolation of exosomes from cultured BMSCs.
  • Culture of HA-VSMCs in control, high phosphorus, and high phosphorus + EXO conditions.
  • Analysis of calcification indicators, calcium content, alkaline phosphatase (AKP) activity, and microRNA (miRNA) and mRNA expression profiles.

Main Results:

  • BMSC-derived exosomes inhibited high phosphorus-induced calcification in HA-VSMCs.
  • EXO treatment reduced calcium content and AKP activity in calcified HA-VSMCs.
  • EXO treatment significantly altered miRNA and mRNA expression, implicating mTOR, MAPK, and Wnt signaling pathways.

Conclusions:

  • BMSC-derived exosomes effectively alleviate vascular calcification in HA-VSMCs.
  • The therapeutic effect of EXO involves the modulation of miRNA profiles and associated signaling pathways.
  • EXO represent a promising therapeutic agent for vascular calcification in CKD.

Keywords:
Bone mesenchymal stem cellsChronic kidney diseaseExosomesMicroRNAVascular calcification

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