Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural homologies among the hemopoietins.

J W Schrader, H J Ziltener, K B Leslie

    Proceedings of the National Academy of Sciences of the United States of America
    |April 1, 1986
    PubMed
    Summary

    Cytokines targeting hematopoietic stem cells share N-terminal amino acid sequence similarities. This homology may serve as a structural marker for this cytokine group.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Stem cell differentiation in the bone-marrow.

    Immunology today·2014
    Same author

    Pandemic H1N1 Influenza Infection and Vaccination in Humans Induces Cross-Protective Antibodies that Target the Hemagglutinin Stem.

    Frontiers in immunology·2012
    Same author

    Expression of activated M-Ras in hemopoietic stem cells initiates leukemogenic transformation, immortalization and preferential generation of mast cells.

    Oncogene·2006
    Same author

    Biased immunoglobulin G (IgG) subclass production in a case of hyper-IgM syndrome.

    Clinical and diagnostic laboratory immunology·2004
    Same author

    The p38 mitogen-activated protein kinases can have opposing roles in the antigen-dependent or endotoxin-stimulated production of IL-12 and IFN-gamma.

    European journal of immunology·2001
    Same author

    IL-2, -4, and -15 differentially regulate O-glycan branching and P-selectin ligand formation in activated CD8 T cells.

    Journal of immunology (Baltimore, Md. : 1950)·2001

    Area of Science:

    • Hematology
    • Molecular Biology
    • Immunology

    Background:

    • Cytokines are crucial signaling molecules.
    • Hematopoietic stem cells (HSCs) are vital for blood cell production.
    • Specific cytokines target HSCs and their derivatives.

    Purpose of the Study:

    • To investigate shared characteristics among cytokines targeting HSCs.
    • To identify potential structural markers for this cytokine group.

    Main Methods:

    • Comparative analysis of N-terminal amino acid sequences of various cytokines.
    • Identification of conserved amino acid residues and patterns.

    Main Results:

    • Several cytokines, including Interleukin-2 (IL-2) and Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), exhibit N-terminal amino acid sequence homology.
    • A conserved alanine residue at the N-terminus and similarities in the subsequent amino acids were observed.
    • Extensive homology was noted between GM-CSF and IL-2.
    • Colony-stimulating factor 1 also shows a similar N-terminal pattern.

    Conclusions:

    • N-terminal amino acid sequence homology serves as a potential structural marker for cytokines acting on HSCs.
    • This finding aids in classifying and understanding the functional relationships of these cytokines.
    • The evolutionary origin of this homology requires further investigation.

    Related Experiment Videos