Visualized detection of apo-transferrin based on cyanine dye supramolecular assembly

In this work, a new biological probe for human transferrin (hTf) detection based on a cyanine dye ETC supramolecular assembly was designed. In sub-micromolar level, apo-hTf could induced the ETC aggregations transferred from H-aggregations to J-band with color change from pink to blue, while holo-hTf hardly possessed the ability, indicating ETC could specifically identify apo-hTf. The present study allowed for apo-hTf detection in the range of 8-80 nM with a detection limit of 2.8 nM and the sensitivity of visualization was around 70 nM. To further examine the suitability, iron ions were added into apo-hTf to stimulate the transformation from open conformation to the closed one gradually. It has been confirmed through tryptophan internal fluorescence quenching and the decrease of ETC J-aggregation. The interaction between ETC and apo-hTf performed high affinity that K_a was reached to 10⁶ M^-1 with a high selectivity. The potential in practical applications of this method has been tested for detection apo-hTf in human serum.