Integrative process for sugarcane bagasse biorefinery to co-produce xylooligosaccharides and gluconic acid

An integrated and green process for co-producing xylooligosaccharides (XOS) and gluconic acid (GA), was developed by utilizing sugarcane bagasse as starting material. In this study, the highest XOS yield of 39.1% obtained from the prehydrolysis was achieved with 10% acetic acid at 150 °C for 45 min. Subsequently, 88.6% conversion of cellulose was achieved in a fed-batch enzymatic hydrolysis using a solid loading of 15%. Results of glucose fermentation suggested that inherent regulatory system of strain Gluconobacter oxydans ATCC 621H boosted GA accumulation without the requirement of pH control, leading to a good 96.3% of GA yield. Great performance of this strain offer an economically feasible option for the large-scale sustainable GA production from biomass. Overall, approximately 105 g XOS and 340 g GA were co-produced from 1 kg of dried sugarcane bagasse as feedstock; this integrated process might be a cost-effective option for the comprehensive utilization of sugarcane bagasse.