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Sampling Methods: Sample Types01:18

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Sampling materials are classified into three main types: solid, liquid, and gas.
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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Convenience Sampling Method00:55

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Sampling is a technique to select a portion (or subset) of the larger population and study that portion (the sample) to gain information about the population. Data are the result of sampling from a population. The sampling method ensures that samples are drawn without bias and accurately represent the population.
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A sample refers to a smaller subset representative of a larger population. In analytical chemistry, studying or analyzing an entire population is often impractical or impossible. Therefore, samples are used to draw inferences and generalize the whole population. The sampling method selects individuals or items from a population to create a sample. Standard sampling methods include random, judgemental, systematic, stratified, and cluster sampling. 
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Systematic Sampling Method01:17

Systematic Sampling Method

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Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
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Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics.

Leslie Muller1, Luc Fornecker1, Sarah Cianferani1

  • 1Laboratoire de Spectrométrie de Masse Bio-Organique (LSMBO), IPHC, UMR 7178, Université de Strasbourg, CNRS, Strasbourg, France.

Methods in Molecular Biology (Clifton, N.J.)
|March 11, 2019
PubMed
Summary

Tube-gel (TG) is a rapid proteomics sample preparation method. This technique streamlines workflows by avoiding electrophoresis, making it ideal for high-throughput quantitative proteomics and biomarker discovery.

Keywords:
Detergent-compatibilityHigh-throughputQuantitative proteomicsSample preparationTube-gel

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Area of Science:

  • Proteomics
  • Biochemistry
  • Analytical Chemistry

Background:

  • Sample preparation is critical for proteomics.
  • Traditional methods can be time-consuming and labor-intensive.
  • High-throughput analysis demands efficient preparation techniques.

Purpose of the Study:

  • To introduce and evaluate the Tube-gel (TG) method for proteomics sample preparation.
  • To highlight the advantages of TG for high-throughput quantitative proteomics.

Main Methods:

  • Tube-gel (TG) involves instantaneous trapping of samples in a polyacrylamide gel matrix.
  • TG utilizes in-gel sample preparation advantages, including high sodium-dodecyl sulfate concentrations.
  • Electrophoresis step is omitted to reduce processing time.

Main Results:

  • TG is a fast and repeatable sample preparation method.
  • The method minimizes sample handling, suitable for large sample numbers.
  • TG is particularly applicable to biomarker research and clinical proteomics.

Conclusions:

  • Tube-gel (TG) offers an efficient alternative for proteomics sample preparation.
  • Its speed and repeatability make it valuable for high-throughput quantitative studies.
  • TG facilitates biomarker discovery and clinical proteomics projects.