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Related Experiment Video

Updated: Jan 28, 2026

Laminectomy for the Removal of Thoracic Ossification of the Ligamentum Flavum TOLF Using Ultrasonic and Conventional Osteotomes
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Cdc42 regulates cranial suture morphogenesis and ossification.

Ryo Aizawa1, Atsushi Yamada2, Tatsuaki Seki3

  • 1Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo, Japan; Department of Periodontology, School of Dentistry, Showa University, Ohta, Tokyo, Japan.

Biochemical and Biophysical Research Communications
|March 12, 2019
PubMed
Summary

Cell division cycle 42 (Cdc42) is vital for cranial bone development. Loss of Cdc42 impairs intramembranous ossification by affecting Indian hedgehog and bone morphogenetic protein signaling pathways.

Keywords:
Cdc42Conditional knockout miceOsteogenesisSuture

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Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Cdc42 (cell division cycle 42) is a small GTPase in the Rho protein family.
  • Previous studies generated Cdc42 conditional knockout mice (Cdc42fl/fl; Prx1-Cre) exhibiting limb and cranial bone deformities, but the cranial mechanism was unknown.

Purpose of the Study:

  • To investigate the role of Cdc42 in cranial bone development.
  • To elucidate the molecular mechanisms underlying Cdc42's function in osteogenesis.

Main Methods:

  • Utilized Cdc42 conditional knockout mice (Cdc42fl/fl; Prx1-Cre) to study cranial bone development.
  • Analyzed gene expression related to cranial suture morphogenesis.

Main Results:

  • Loss of Cdc42 resulted in defective intramembranous ossification in cranial bone.
  • Observed decreased expression of key cranial suture morphogenesis genes, including Indian hedgehog (Ihh) and bone morphogenetic proteins (BMPs).

Conclusions:

  • Cdc42 plays a critical role in cranial osteogenesis.
  • Cdc42-mediated cranial development is regulated by Ihh- and BMP-signaling pathways.