Related Experiment Video
Updated: Jan 28, 2026
11:32
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
Published on: May 24, 2017
12.6K
Mapping In Vivo RNA Structures and Interactions
Jieyu Zhao1, Xingyang Qian2, Pui Yan Yeung1
1Department of Chemistry, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China; These authors contributed equally to this work.
Trends in Biochemical Sciences
|March 12, 2019
Summary
Researchers explore technologies for mapping RNA structures and interactions within cells. These methods are crucial for understanding RNA
Area of Science:
- Molecular Biology
- Biochemistry
- Genetics
Background:
- RNA molecules fold into complex secondary and tertiary structures.
- RNA interacts with various biomolecules to perform cellular functions.
- Understanding in vivo RNA structure and interactions is vital for cell biology.
Purpose of the Study:
- To classify and review existing technologies for mapping RNA structures.
- To categorize methods for analyzing in vivo RNA-protein interactions.
- To provide an overview of current RNA structure-mapping approaches.
Main Methods:
- Literature review and classification of RNA structure-mapping technologies.
- Categorization based on the principles and applications of each technology.
- Analysis of methods for probing RNA conformation and interactions in living cells.
Main Results:
- Technologies for mapping RNA structures and interactions fall into four broad categories.
- Each category encompasses distinct methodologies for analyzing RNA in its cellular context.
- The reviewed technologies offer diverse approaches to studying RNA folding and binding.
Conclusions:
- A comprehensive classification of in vivo RNA structure-mapping technologies is presented.
- This categorization aids in understanding the landscape of available research tools.
- Further development in these areas will enhance our knowledge of RNA function.
More Related Videos
Related Concept Videos
01:23RNA Structure
79.0K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.0K
01:19RNA Structure
7.5K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
7.5K
02:41Chromatin Structure and RNA Splicing
3.4K
3.4K
01:53RNA Stability
35.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.7K
02:36RNA Polymerase II Accessory Proteins
10.9K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
10.9K
00:58Eukaryotic RNA Polymerases
27.0K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
27.0K