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Updated: Jan 28, 2026

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
Published on: August 14, 2009
Quantitation of low concentrations of polysorbates 80 in protein formulations by Coomassie brilliant blue
Yongfeng Cheng1, Yuling Liu1, Haiming Wei1
1Division of Molecular Medicine, Hefei National Laboratory for Physical Sciences at Microscale, the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China; Institue of Immunology, University of Science and Technology of China, Hefei, Anhui, China.
Abstract:
Polysorbate 80, as pharmaceutical excipient and virus inactivating agent, is commonly used in the protein pharmaceutical industry. In this study, a method has been developed for the determination of low concentration of Polysorbate 80 in the presence of high concentration proteins (≤100 mg/ml) and excipients. This colorimetric method is based on the interaction of Polysorbate 80 and Coomassie brilliant blue, and suitable for quantitation of Polysorbate 80 in the range of 10-100 μg/ml. Dozens or hundreds of samples can be quantified simultaneously by using microplate. Besides Polysorbate 80, this method can also be used to determine other types of surfactants in protein solutions, such as Polysorbate 20, Triton X-100, NP40, SDS, Benzalkonium chloride/bromide and PEG4000.
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