Related Experiment Video
Updated: Jan 28, 2026
09:54
Laboratory Scale Production and Purification of a Therapeutic Antibody
Published on: January 24, 2017
18.3K
Purification of caprine oligosaccharides at pilot-scale
Leticia F M C Aquino1, Juliana M L N de Moura Bell1,2, Joshua L Cohen1
1Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis, CA 95616, United States.
Journal of Food Engineering
|March 12, 2019
Summary
This study optimized caprine milk oligosaccharide (COS) purification by combining lactose hydrolysis, fermentation, and nanofiltration. This integrated approach achieved high COS recovery yields with minimal lactose contamination, improving purity for valuable goat milk components.
Area of Science:
- Food Science
- Biotechnology
- Analytical Chemistry
Background:
- Caprine milk oligosaccharides (COS) are valuable bioactive compounds.
- Purification challenges include low COS concentration, high lactose levels, and similar molecular weights.
- Existing membrane filtration methods struggle with purity and yield.
Purpose of the Study:
- To develop an optimized, integrated method for high-purity COS concentrate recovery from caprine milk.
- To overcome limitations of traditional membrane filtration for COS purification.
- To achieve high recovery yields of COS with minimal lactose contamination.
Main Methods:
- Integrated approach: lactose hydrolysis, monosaccharide fermentation, and nanofiltration.
- Ultrafiltration with discontinuous diafiltration of defatted goat whey.
- Concentration of whey permeate using nanofiltration.
- Quantification of carbohydrates via High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC PAD).
Main Results:
- Achieved 75% COS recovery yields.
- Reduced monosaccharides to negligible levels (0.3% of initial lactose).
- Obtained a final retentate with 67.6% acidic and 34.4% neutral oligosaccharides.
Conclusions:
- The integrated approach effectively purifies COS from caprine milk.
- This method overcomes challenges associated with lactose and molecular weight similarity.
- The optimized process yields a high-purity COS concentrate suitable for further applications.
Related Concept Videos
01:24Oligosaccharide Assembly
3.6K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
3.6K
01:21Pilot and Numeric Relaying
487
Pilot relaying is a type of differential protection used in power systems. It compares electrical quantities at the terminals of equipment via a communication channel instead of direct relay interconnection. This method is essential for transmission lines where the terminals are far apart, typically up to 80 km for lines with 69 to 115 kV ratings. Four types of communication channels are used for pilot relaying:
487
02:41pH Scale
79.4K
Hydronium and hydroxide ions are present both in pure water and in all aqueous solutions, and their concentrations are inversely proportional as determined by the ion product of water (Kw). The concentrations of these ions in a solution are often critical determinants of the solution’s properties and the chemical behaviors of its other solutes. Two different solutions can differ in their hydronium or hydroxide ion concentrations by a million, billion, or even trillion times. A common means of...
79.4K
01:26Scaling
592
In designing and analyzing filters, resonant circuits, or circuit analysis at large, working with standard element values like 1 ohm, 1 henry, or 1 farad can be convenient before scaling these values to more realistic figures. This approach is widely utilized by not employing realistic element values in numerous examples and problems; it simplifies mastering circuit analysis through convenient component values. The complexity of calculations is thereby reduced, with the understanding that...
592
01:22Thermometers and Temperature Scales
7.5K
Any physical property that depends consistently and reproducibly on temperature can be used as the basis of a thermometer. For example, volume increases with temperature for most substances. This property is the basis for the common alcohol thermometer and the original mercury thermometers. Other properties used to measure temperature include electrical resistance, color, and the emission of infrared radiation.
As many physical properties depend on temperature, the variety of thermometers is...
As many physical properties depend on temperature, the variety of thermometers is...
7.5K
01:22Gas Thermometers and the Kelvin Scale
6.2K
The definition of temperature in terms of molecular motion suggests that there should be a lowest possible temperature, where the average kinetic energy of molecules is zero (or the minimum allowed by quantum mechanics). Experiments confirm the existence of such a temperature, called absolute zero. An absolute temperature scale is one whose zero point is absolute zero. Such scales are convenient in science because several physical quantities, such as the volume of an ideal gas, are directly...
6.2K