Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Fast Fourier Transform

Fast Fourier Transform

The Fast Fourier Transform (FFT) is a computational algorithm designed to compute the Discrete Fourier Transform (DFT) efficiently. By breaking down the calculations into smaller, manageable sections, the FFT significantly reduces the computational complexity involved. Direct computation of an N-point DFT requires N2 complex multiplications, whereas the FFT algorithm needs only (N/2)log⁡2N multiplications, offering a much faster performance.
The computational efficiency of the FFT becomes...

Composition of Blood Plasma

Composition of Blood Plasma

Blood plasma is a fluid that contains approximately 92% water and 8% solutes. The solutes include various types of proteins, which constitute about 7% of the total solutes in the plasma. The high-molecular-weight proteins—albumins, globulins, and fibrinogen—are essential to plasma function. Albumins, making up about 60% of the plasma proteins, maintain the osmotic balance within blood vessels by preventing excessive water leakage. Additionally, albumins serve as carrier proteins,...

Gene Evolution - Fast or Slow?

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...

Gene Evolution - Fast or Slow?

Gene Evolution - Fast or Slow?

Fast Decoupled and DC Powerflow

Fast Decoupled and DC Powerflow

The fast decoupled power flow method addresses contingencies in power system operations, such as generator outages or transmission line failures. This method provides quick power flow solutions, essential for real-time system adjustments. Fast decoupled power flow algorithms simplify the Jacobian matrix by neglecting certain elements, leading to two sets of decoupled equations:

Simple Pendulum

Simple Pendulum

A simple pendulum consists of a small diameter ball suspended from a string, which has negligible mass but is strong enough to not stretch. In our daily life, pendulums have many uses, such as in clocks, on a swing set, and on a sinker on a fishing line.
The period of a simple pendulum depends on two factors: its length and the acceleration due to gravity. The period is completely independent of any other factors, such as mass or maximum displacement. For small displacements, a pendulum is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Strategies to Overcome Hematocrit and Volume Bias in Dried Blood Spot Analysis.

Pharmaceuticals (Basel, Switzerland)·2026

Same author

Development, Fabrication and Application of a Sectioned 3D-Printed Human Nasal Cavity Model for In Vitro Nasal Spray Deposition Studies.

Biomedicines·2026

Same author

Extractables and Leachables in Pharmaceutical Products: Potential Adverse Effects and Toxicological Risk Assessment.

Toxics·2026

Same author

Assessment of Sedation in Mechanically Ventilated Children with Severe Acute Bronchiolitis: Correlation Between COMFORT-B Scale and Bispectral Index During Continuous Infusion of Fentanyl and Midazolam.

Medicina (Kaunas, Lithuania)·2025

Same author

Oxidative Stress-Related Biomarkers in Inflammatory Bowel Disease: Dual Tools for Remission Assessment and Prediction of Treatment Outcome.

Antioxidants (Basel, Switzerland)·2025

Same author

Development, Validation and Application of the Dried Blood Spot Analysis Method for the Determination of Ustekinumab in Patients with Inflammatory Bowel Disease.

Pharmaceuticals (Basel, Switzerland)·2025

Search research articles

Related Experiment Video

Updated: Jan 28, 2026

A Simple Double Centrifugation Tube Method to Obtain Platelet-rich Plasma from Equine Blood

A Simple Double Centrifugation Tube Method to Obtain Platelet-rich Plasma from Equine Blood

Published on: August 15, 2025

Fast and Simple LC-MS/MS Method for Rifampicin Quantification in Human Plasma.

Žane Temova Rakuša¹, Robert Roškar¹, Anita Klančar Andrejc¹

¹University of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia.

International Journal of Analytical Chemistry

|March 12, 2019

Summary

A new LC-MS/MS method rapidly quantifies rifampicin in human plasma. This cost-effective assay is ideal for therapeutic drug monitoring and pharmacokinetic studies.

More Related Videos

Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites

Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites

Published on: March 16, 2018

Detection and Quantification of Calcitonin Gene-Related Peptide CGRP in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Detection and Quantification of Calcitonin Gene-Related Peptide CGRP in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Published on: June 16, 2023

Related Experiment Videos

Last Updated: Jan 28, 2026

A Simple Double Centrifugation Tube Method to Obtain Platelet-rich Plasma from Equine Blood

A Simple Double Centrifugation Tube Method to Obtain Platelet-rich Plasma from Equine Blood

Published on: August 15, 2025

Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites

Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites

Published on: March 16, 2018

Detection and Quantification of Calcitonin Gene-Related Peptide CGRP in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Detection and Quantification of Calcitonin Gene-Related Peptide CGRP in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

Published on: June 16, 2023

Area of Science:

Analytical Chemistry
Pharmacology

Background:

Rifampicin is a crucial antibiotic for treating tuberculosis.
Accurate quantification of rifampicin in plasma is essential for effective treatment and pharmacokinetic studies.

Purpose of the Study:

To develop and validate a simple, fast, and cost-effective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for rifampicin quantification in human plasma.

Main Methods:

Utilized a Captiva ND Lipids filtration plate for sample cleanup.
Employed a 1290 Infinity liquid chromatograph with a 6460 Triple Quadrupole mass spectrometer.
Achieved chromatographic separation on a core-shell Kinetex C18 column using gradient elution.

Main Results:

Developed the fastest method to date for rifampicin analysis, with sample prep around 1 minute and a total run time of 2.4 minutes.
Demonstrated outstanding sensitivity and a wide analytical range (5-40000 μg/L) with excellent linearity, accuracy, and precision.
Achieved high recovery (92%) and minimal matrix effects, validated across plasma samples from multiple volunteers.

Conclusions:

The validated LC-MS/MS method is highly suitable for therapeutic drug monitoring and pharmacokinetic analysis of rifampicin.
Its speed, sensitivity, and cost-effectiveness make it a valuable tool for clinical applications.
Successfully applied to quantify rifampicin in 340 patient plasma samples, confirming its practical utility.