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Induction and Analysis of Epithelial to Mesenchymal Transition
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Annexin A2 Silencing Inhibits Proliferation and Epithelial-to-mesenchymal Transition through p53-Dependent Pathway in

Minhua Wu1, Yanqin Sun2, Feipeng Xu3

  • 1Department of Histology and Embryology, Guangdong Medical University, Zhanjiang 524023, Guangdong, China.

Journal of Cancer
|March 12, 2019
PubMed

Insights

Annexin A2 is overexpressed in non-small cell lung cancers (NSCLCs), promoting tumor progression and metastasis. Reducing Annexin A2 inhibits NSCLC growth and invasion, suggesting it as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Annexin A2 is implicated in cancer cell adhesion, invasion, and metastasis.
  • The precise role and mechanism of Annexin A2 in non-small cell lung cancer (NSCLC) progression remain unclear.

Purpose of the Study:

  • To investigate the function and mechanism of Annexin A2 in NSCLC tumor progression.
  • To evaluate Annexin A2 as a potential prognostic marker and therapeutic target for NSCLC.

Main Methods:

  • Quantitative analysis of Annexin A2 expression in NSCLC cell lines and tissues.
  • Correlation analysis between Annexin A2 expression and clinicopathological features.
  • Kaplan-Meier survival analysis.
  • In vitro knockdown experiments of Annexin A2.
  • Western blot analysis to assess p53 expression, cell cycle, and epithelial-to-mesenchymal transition (EMT) markers.

Main Results:

  • Annexin A2 was significantly overexpressed in NSCLC tissues and cell lines.
  • High Annexin A2 expression correlated with advanced clinical stage and lymph node metastasis.
  • Patients with high Annexin A2 expression exhibited poorer overall survival.
  • Knockdown of Annexin A2 suppressed NSCLC cell proliferation and invasion.
  • Annexin A2 knockdown led to increased p53 expression, G2 cell cycle arrest, and inhibition of EMT.

Conclusions:

  • Annexin A2 plays a crucial role in NSCLC progression.
  • Annexin A2 serves as a potential prognostic biomarker for NSCLC.
  • Annexin A2 represents a novel therapeutic target for NSCLC treatment.
Keywords:
Annexin A2NSCLCscell proliferationepithelial-to-mesenchymal transitionp53

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