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CMAH genotyping survey for blood types A, B and C (AB) in purpose-bred cats

A Kehl1, E Mueller1, U Giger2

  • 1Laboklin, Steubenstraße 4, Bad Kissingen, D-97688, Germany.

Animal Genetics
|March 12, 2019
PubMed

Insights

A new genotyping scheme accurately identifies domestic cat blood types A, B, and C, preventing transfusion reactions and neonatal isoerythrolysis in kittens. This method improves upon existing panels for breeders and veterinarians.

Area of Science:

  • Veterinary Genetics
  • Animal Blood Groups
  • Molecular Diagnostics

Background:

  • The domestic cat AB blood group system comprises types A, B, and C (AB), with potential for severe transfusion reactions and neonatal isoerythrolysis (NI) due to incompatibilities.
  • Blood types B and C are recessive, necessitating accurate genotyping for breeders to predict offspring blood types and prevent NI.
  • Existing genotyping schemes for feline blood types have limitations, prompting the need for improved diagnostic tools.

Purpose of the Study:

  • To develop and validate a superior single nucleotide variant (SNV) genotyping panel for accurate feline blood type determination (A, B, and C).
  • To identify novel breed-specific SNVs associated with feline blood group alleles.
  • To enhance the ability of veterinarians and breeders to prevent adverse transfusion events and NI in cats.

Main Methods:

  • Genotyping of 2145 domestic cats using both an original SNV panel and a newly developed panel.
  • The new panel incorporates specific SNVs (c.179G>T, c.268T>A, c.1322delT) to differentiate types A and B, and a SNV (c.364C>T) for the ac allele.
  • Comparison of genotyping results between the original and new SNV panels to assess accuracy and identify new variants.

Main Results:

  • The new SNV panel accurately genotyped all 2145 cats, assigning correct blood types A, B, and C.
  • Novel breed-specific SNVs (c.179G>T, c.1322delT) for the b allele were identified in several breeds.
  • The new scheme successfully differentiated genotypes including A/ac (type A), ac /ac (C), and ac /b (C), and detected the ac variant in additional breeds.

Conclusions:

  • The developed SNV panel offers superior performance for feline blood type genotyping compared to the original panel.
  • Accurate identification of blood types A, B, and C is crucial for preventing hemolytic transfusion reactions and NI.
  • This improved genotyping method provides essential support for veterinary clinicians and cat breeders in managing blood incompatibilities.

Keywords:
blood group systemsblood typingcytidine monophosphate-N-acetylneuraminic acid hydroxylasefelinesingle nucleotide polymorphisms

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