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A versatile liquid-jet setup for the European XFEL
J Schulz1, J Bielecki1, R B Doak2
1European XFEL, Holzkoppel 4, Schenefeld 22869, Germany.
Journal of Synchrotron Radiation
|March 12, 2019
Summary
The European XFEL
Area of Science:
- Structural Biology
- X-ray Crystallography
- Biophysics
Background:
- High-throughput serial femtosecond crystallography (SFX) requires advanced sample delivery systems.
- The SPB/SFX instrument at the European XFEL enables novel crystallography experiments.
- Efficient sample delivery is crucial for maximizing data collection in SFX.
Purpose of the Study:
- To present the novel liquid-jet sample delivery setup for the SPB/SFX instrument.
- To detail the capabilities and compatibility of the new sample delivery system.
- To demonstrate visual control mechanisms for the liquid jets.
Main Methods:
- Implementation of a versatile liquid-jet system compatible with gas dynamic virtual nozzles and high-viscosity extruders.
- Integration of a differentially pumped catcher assembly for jet confinement.
- Utilization of a dual-microscope imaging system for real-time jet visualization.
Main Results:
- The liquid-jet setup supports various sample delivery devices, including state-of-the-art systems.
- Rapid sample jet replacement (within minutes) is achievable via a load-lock mechanism.
- The two-microscope system provides comprehensive visual monitoring of the liquid jets.
Conclusions:
- The developed liquid-jet sample delivery system enhances the capabilities of the SPB/SFX instrument for high-throughput SFX.
- The system's flexibility and rapid exchange facilitate efficient experimental workflows.
- This setup is a key advancement for serial femtosecond crystallography at the European XFEL.
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