Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A fluorometric assay for biotinidase.

H Ebrahim, K Dakshinamurti

    Analytical Biochemistry
    |April 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    A new fluorometric assay accurately measures biotinidase activity using its natural substrate, biocytin. This method allows direct determination of lysine, improving biotinidase enzyme analysis in human serum.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Functional Characterization of <i>ycao</i> in <i>Escherichia coli</i> C91 Reveals Its Role in Siderophore Production, Iron-Limited Growth, and Antimicrobial Activity.

    Antibiotics (Basel, Switzerland)·2026
    Same author

    Genotypic and phenotypic analyses of two distinct sets of <i>Pseudomonas aeruginosa</i> urinary tract isolates.

    Journal of medical microbiology·2025
    Same author

    Phenotype and biochemical heterogeneity in late onset Fabry disease defined by N215S mutation.

    PloS one·2018
    Same author

    Blood pressure regulation and micronutrients.

    Nutrition research reviews·2008
    Same author

    Wrapped branes as qubits.

    Physical review letters·2008
    Same author

    Interleukin-4 and interleukin-13: bidirectional effects on human osteoclast formation.

    Bone·2001

    Area of Science:

    • Biochemistry
    • Enzymology
    • Analytical Chemistry

    Background:

    • Biotinidase is a crucial enzyme in biotin metabolism.
    • Accurate assays are needed for diagnosing biotinidase deficiencies.

    Purpose of the Study:

    • To describe a novel fluorometric assay for biotinidase activity.
    • To validate the assay using biocytin as the natural substrate.
    • To assess the assay's applicability in human serum samples.

    Main Methods:

    • Development of a fluorometric assay utilizing 1,2-diacetylbenzene.
    • Selective reaction of 1,2-diacetylbenzene with lysine.
    • Direct determination of lysine in the presence of biocytin.
    • Application and testing of the assay with human serum biotinidase.

    Related Experiment Videos

    Main Results:

    • The assay successfully measures biotinidase activity using biocytin.
    • 1,2-diacetylbenzene allows for selective lysine determination.
    • The assay is applicable for analyzing human serum biotinidase.

    Conclusions:

    • A sensitive and specific fluorometric assay for biotinidase has been developed.
    • This assay enables direct measurement of enzyme activity via its natural substrate.
    • The method provides a valuable tool for clinical diagnostics and research.