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Towards integrated multi-sensor platform using dual electrochemical and optical detection for on-site pollutant
F Sekli Belaïdi1, L Farouil1, L Salvagnac1
1CNRS, LAAS, 7 Avenue du Colonel Roche, F-31400 Toulouse, France; Université de Toulouse, UPS, LAAS, F-31400 Toulouse, France.
Biosensors & Bioelectronics
|March 12, 2019
Summary
This study developed a lab-on-chip device for water toxicity analysis, specifically detecting herbicides by monitoring algal metabolism. The device simultaneously measures fluorescence and oxygen production, enabling sensitive, on-site pollutant detection without sample preparation.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Biotechnology
Background:
- Water toxicity analysis requires sensitive and rapid detection methods for environmental monitoring.
- Current methods for herbicide detection can be time-consuming and require sample pre-treatment.
- Algal metabolism monitoring offers a sensitive indicator of water quality and pollutant effects.
Purpose of the Study:
- To develop an integrated lab-on-chip (LOC) device for simultaneous electrochemical and optical detection.
- To monitor algal metabolism for environmental water toxicity and herbicide analysis.
- To assess the efficacy of the LOC device for on-site pollutant detection without sample preparation.
Main Methods:
- Functional integration of three-electrode electrochemical microcells (ElecCell) and organic photodetectors (OPD) onto a LOC platform.
- Simultaneous measurement of microalgae fluorescence and oxygen production using three species: Chlamydomonas reinhardtii, Pseudokirchneriella subcapitata, and Chlorella vulgaris.
- Application of the multi-microsensor platform to study the effects of Diuron herbicide on algal photosynthesis and metabolism.
Main Results:
- The LOC device successfully monitored variations in microalgae fluorescence and oxygen production.
- Diuron herbicide exposure resulted in fluorescence enhancement and oxygen production inhibition at low nanomolar concentrations.
- Measurements were performed on both unconcentrated and concentrated algae solutions, demonstrating the system's ability to work without sample pre-treatment.
Conclusions:
- The developed LOC device provides a robust platform for dual electrochemical/optical detection.
- The system is highly effective for on-site water pollutant analysis, including herbicide detection, without sample pre-treatment.
- This technology advances environmental monitoring capabilities for water toxicity assessment.
Keywords:
Compact fluidic platformElectrochemical detectionHerbicide detectionLED excitationOrganic photo-detection.algal metabolismMore Related Videos
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