Related Experiment Video
Updated: Jan 28, 2026
06:38
Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
Published on: November 8, 2011
41.2K
The BOLF1 gene is necessary for effective Epstein-Barr viral infectivity.
H M Abdullah Al Masud1, Takahiro Watanabe1, Yoshitaka Sato1
1Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Virology
|March 12, 2019
Summary
The Epstein-Barr virus (EBV) protein BOLF1 is crucial for enhancing viral infectivity by aiding nuclear transport of incoming viruses. Its absence significantly reduces EBV
Area of Science:
- Virology
- Molecular Biology
Background:
- Epstein-Barr virus (EBV) causes infectious mononucleosis and cancers.
- BOLF1 is a conserved EBV lytic tegument protein.
Purpose of the Study:
- Investigate the role of EBV BOLF1 protein in viral replication and infectivity.
- Determine BOLF1's function in the viral lytic cycle.
Main Methods:
- Constructed BOLF1-deficient EBV using BAC and CRISPR/Cas9.
- Assessed viral protein expression, DNA synthesis, and progeny release.
- Analyzed viral nuclear transport and infectivity.
Main Results:
- BOLF1 deficiency minimally impacted viral protein expression, DNA synthesis, and progeny release.
- Loss of BOLF1 significantly reduced EBV infectivity.
- Disruption of BOLF1 decreased nuclear transport of incoming EBV particles.
- BOLF1 interacts with BKRF4 and both localize to the nucleus/perinuclear area during the lytic cycle.
Conclusions:
- BOLF1 enhances EBV progeny virion infectivity.
- BOLF1 facilitates nuclear transport of incoming nucleocapsids.
- BOLF1 and BKRF4 play roles in nuclear localization during the EBV lytic cycle.
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