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Defining Substrate Specificities for Lipase and Phospholipase Candidates
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Factors Affecting Lipase Production by Mucor racemosus.

A K Chopra1, Harish Chander1, V K Batish1

  • 1Division of Dairy Bacteriology, National Dairy Research Institute, Karnal (Haryana) 132001, India.

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|March 13, 2019
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Summary

This study optimized lipase production from Mucor racemosus, identifying key factors like temperature, pH, and nutrient supplementation for enhanced enzyme yield.

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Area of Science:

  • Microbiology
  • Enzymology
  • Biotechnology

Background:

  • Lipases are crucial enzymes with diverse industrial applications.
  • Optimizing microbial lipase production is essential for cost-effective enzyme manufacturing.
  • Mucor racemosus is a known lipase-producing fungus.

Purpose of the Study:

  • To determine optimal conditions for lipase production by Mucor racemosus.
  • To investigate the effect of medium composition on enzyme yield.
  • To identify factors stimulating lipase production.

Main Methods:

  • Culturing Mucor racemosus under various static conditions.
  • Testing different temperatures, pH levels, and incubation times.
  • Supplementing the growth medium with various carbon sources (glucose, lactose), nitrogen sources (peptone), and citrate salts.

Main Results:

  • Maximum lipase production was achieved at 22°C, pH 5.0, and 3 days of static culture.
  • Glucose supplementation (1%) yielded the highest enzyme production (18.0 μmoles FFA).
  • Peptone (2%) as a nitrogen source and citrate salts significantly stimulated lipase production (up to 80%).

Conclusions:

  • Optimal conditions for Mucor racemosus lipase production were established.
  • Medium supplementation, particularly with glucose and peptone, is critical for maximizing enzyme yield.
  • Citrate salts act as potent stimulators for lipase production in this fungal species.