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Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
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Inoculation of Tomato Spotted Wilt Virus into Cotton
R L Groves1, G G Kennedy1, J F Walgenbach1
1Department of Entomology, North Carolina State University, Raleigh 27695.
Plant Disease
|March 13, 2019
Summary
Cotton is susceptible to Tomato spotted wilt virus (TSWV), with the one-leaf stage showing the highest infection rates. This finding highlights cotton
Area of Science:
- Plant Pathology
- Virology
- Entomology
Background:
- Tomato spotted wilt virus (TSWV) poses a significant economic threat to global agriculture.
- Cotton (Gossypium hirsutum L.) is a known host for TSWV and an early-season reservoir for its thrips vectors.
Purpose of the Study:
- To evaluate the susceptibility of commercial cotton varieties to TSWV.
- To determine the susceptibility of cotton at different growth stages to TSWV transmission by tobacco thrips (Frankliniella fusca).
Main Methods:
- Four cotton varieties were mechanically inoculated with four TSWV isolates.
- Virus recovery was confirmed using Emilia sonchifolia as an indicator plant and DAS-ELISA.
- Cotton susceptibility was assessed at cotyledon, one-leaf, and four-leaf stages for thrips transmission.
Main Results:
- Mean TSWV infection across varieties and isolates was 27% at 21 days post-inoculation.
- TSWV was successfully recovered from infected cotton leaves in 83% of indicator plants.
- Cotton showed highest susceptibility to thrips-transmitted TSWV in the one-leaf stage (63% infection).
Conclusions:
- Cotton varieties are susceptible to TSWV infection through mechanical inoculation and thrips transmission.
- The one-leaf developmental stage of cotton is most vulnerable to TSWV.
- Susceptible cotton may serve as a critical source for TSWV dissemination to other crops.
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