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A Metadata Extraction Approach for Clinical Case Reports to Enable Advanced Understanding of Biomedical Concepts
Published on: September 20, 2018
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First Report of Hibiscus Chlorotic Ringspot Carmovirus in Tuvalu
P Jones1, J Devonshire1, A Dabek2
1Department of Crop & Disease Management, IACR Rothamsted, Harpenden, Herts AL5 2JQ, England.
Plant Disease
|March 13, 2019
Summary
Hibiscus chlorotic ringspot carmovirus (HCRSV) was identified in Tuvalu
Area of Science:
- Plant virology
- Tropical agriculture
Background:
- Hibiscus manihot (nambele) plants on Vaitupu Island, Tuvalu, showed symptoms of angular leaf mosaic and chlorosis.
- This observation prompted an investigation into the causal agent.
Purpose of the Study:
- To identify the virus affecting Hibiscus manihot in Tuvalu.
- To determine the virus's characteristics and potential origin.
Main Methods:
- Electron microscopy was used to examine virus particles from infected leaf sap.
- Poly-acrylamide gel electrophoresis (PAGE) analyzed viral protein.
- Immunosorbent electron microscopy (ISEM) confirmed virus identity using specific antisera.
Main Results:
- Isometric virus particles, 28 nm in diameter, were observed.
- PAGE revealed a single protein band of Mr 38,000, characteristic of carmoviruses.
- ISEM identified the virus as hibiscus chlorotic ringspot carmovirus (HCRSV).
Conclusions:
- HCRSV, previously reported in several countries, is now identified in Tuvalu.
- Introduction via imported infected cuttings for food supplements is suspected.
- Dissemination likely occurred through cuttings and mechanical transmission within the islands.
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