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Related Concept Videos

Formation of Species01:31

Formation of Species

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Speciation describes the formation of one or more new species from one or sometimes multiple original species. The resulting species are discrete from the parent species, and barriers to reproduction will typically exist. There are two primary mechanisms, speciation with and without geographic isolation—allopatric and sympatric speciation, respectively.
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Solution Formation02:16

Solution Formation

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There is no one solvent that can dissolve every type of solute. Some substances that readily dissolve in a certain solvent might be insoluble in a different solvent. A simple way to predict which substances dissolve in which solvent is the phrase "like dissolves like". This means that polar substances, such as salt and sugar, dissolve in a polar substance like water. In contrast, non-polar substances are more soluble in non-polar solvents such as carbon tetrachloride.
This selective...
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Standard Enthalpy of Formation02:37

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Enthalpy changes are typically tabulated for reactions in which both the reactants and products are at the same conditions. A standard state is a commonly accepted set of conditions used as a reference point for the determination of properties under other different conditions. For chemists, the IUPAC standard state refers to materials under a pressure of 1 bar and solutions at 1 M and does not specify a temperature. Many thermochemical tables list values with a standard state of 1 atm. Because...
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A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
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Microtubule Formation01:23

Microtubule Formation

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Microtubules are dynamic structures that undergo continuous assembly and disassembly. They originate from specialized multi-protein complexes known as microtubule organizing centers or MTOCs. Within the MTOC, the point of origin of the microtubule is known as the minus end, while the end radiating outward is the plus end. Microtubules serve two primary functions — the organization of spindle complexes to separate sister chromatids during mitotic or meiotic cell division and the formation...
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Relationship Formation02:12

Relationship Formation

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What do you think is the single most influential factor in determining with whom you become friends and whom you form romantic relationships? You might be surprised to learn that the answer is simple: the people with whom you have the most contact. This most important factor is proximity. You are more likely to be friends with people you have regular contact with. For example, there are decades of research that shows that you are more likely to become friends with people who live in your dorm,...
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Related Experiment Video

Updated: Jan 28, 2026

Microtiter Dish Biofilm Formation Assay
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Rab7a and Mitophagosome Formation.

Esther Hui Na Tan1,2, Bor Luen Tang3,4

  • 1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Singapore. e0145854@u.nus.edu.

Cells
|March 13, 2019
PubMed
Summary
This summary is machine-generated.

Rab7a, a small GTPase, is crucial for mitophagy by guiding vesicle formation around damaged mitochondria. Its activity relies on specific regulators and signaling pathways, expanding its known roles in membrane traffic and autophagy.

Keywords:
Rab7TBC1D15/17TRAF family-associated NF-κB activator binding kinase 1 (TBK1)Tre-2/Bub2/Cdc16 (TBC)1D5autophagymitophagosomemitophagy

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Autophagy Research

Background:

  • Rab7a, a small GTPase, is traditionally known for regulating endocytic traffic and lysosome fusion.
  • Previous studies suggested Rab7a's involvement in autophagosome-lysosome fusion and autolysosome maturation.
  • Emerging evidence highlights Rab7a's novel functions beyond canonical endocytic pathways.

Purpose of the Study:

  • To elucidate the role of Rab7a and its regulators in PTEN-induced kinase 1 (PINK1)-Parkin-mediated mitophagy.
  • To explore the mechanisms by which Rab7a facilitates autophagosome formation around damaged mitochondria.
  • To provide a broadened perspective on the Rab7a network's involvement in mitophagy.

Main Methods:

  • Investigating the necessity of Rab7a cycling, mediated by its guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs).
  • Assessing the dependence of Rab7a's mitophagy function on the retromer complex.
  • Examining the role of TRAF family-associated NF-κB activator binding kinase 1 (TBK1) phosphorylation in regulating Rab7a activity.

Main Results:

  • Rab7a and its regulators are essential for the proper targeting of Atg9a-bearing vesicles during mitophagy.
  • The mitophagosome formation role of Rab7a is contingent upon its cycling process involving GEFs and GAPs.
  • Rab7a's function in mitophagy is further regulated by the retromer complex and TBK1-mediated phosphorylation.

Conclusions:

  • Rab7a plays a critical role in PINK1-Parkin-mediated mitophagy, extending its known functions in cellular trafficking.
  • The Rab7a network, including its regulators and associated signaling pathways, is vital for efficient removal of damaged mitochondria.
  • This study broadens the understanding of Rab7a's involvement in selective autophagy and cellular quality control mechanisms.