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Chronic Constriction Injury of the Rat's Infraorbital Nerve IoN-CCI to Study Trigeminal Neuropathic Pain
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Foxp3 plasmid-encapsulated PLGA nanoparticles attenuate pain behavior in rats with spinal nerve ligation

Juhee Shin1, Yuhua Yin1, Do Kyung Kim2

  • 1Department of Medical Science, Chungnam National University School of Medicine, Daejeon, Republic of Korea; Department of Anatomy, Brain Research Institute, Chungnam National University School of Medicine, Daejeon, Republic of Korea.

Nanomedicine : Nanotechnology, Biology, and Medicine
|March 13, 2019
PubMed

Insights

Foxp3 nanoparticles reduce neuropathic pain by calming overactive microglia, offering potential therapeutic benefits for pain management. This approach targets neuroinflammation to alleviate pain symptoms.

Area of Science:

  • Neuroscience
  • Immunology
  • Pharmacology

Background:

  • Microglia are key players in neuropathic pain.
  • Foxp3 expression in microglia aids tissue repair and resolves neuroinflammation.
  • Foxp3 may offer a therapeutic strategy for neuropathic pain.

Purpose of the Study:

  • To investigate if Foxp3 nanoparticles (Foxp3 NPs) can alleviate neuropathic pain.
  • To determine if Foxp3 NPs inhibit microglia activity to reduce pain.

Main Methods:

  • Prepared Foxp3 NPs using poly (D,L-lactic-co-glycolic acid).
  • Assessed anti-inflammatory effects on lipopolysaccharide-stimulated BV2 cells in vitro.
  • Evaluated Foxp3 NPs' localization to spinal microglia and pain behavior in a rat model of neuropathic pain.

Main Results:

  • Foxp3 NPs demonstrated anti-inflammatory effects in vitro.
  • Foxp3 NPs localized to spinal microglia in vivo.
  • Foxp3 NPs significantly attenuated neuropathic pain behavior in rats for 7 days.
  • Treatment suppressed microglial activity, downregulated pro-nociceptive genes, and upregulated anti-nociceptive genes in the spinal dorsal horn.

Conclusions:

  • Foxp3 NPs effectively relieve neuropathic pain in animal models.
  • The mechanism involves reducing microglia activity and modulating neuroinflammation.
  • Foxp3 NPs hold therapeutic potential for treating neuropathic pain.
Keywords:
Foxp3MicrogliaNeuropathic painPLGA nanoparticlesSpinal nerve ligation

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