Related Experiment Video

Updated: Jan 28, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

9.4K

Control of Klebsiella pneumoniae Infection in Mice by Using Dissolving Microarray Patches Containing Gentamicin

Aoife M Rodgers1, Maelíosa T C McCrudden1, Aaron J Courtenay1

  • 1School of Pharmacy, Medical Biology Centre, Queens University Belfast, Belfast, United Kingdom.

Antimicrobial Agents and Chemotherapy
|March 13, 2019
PubMed

Insights

Gentamicin dissolving microarray patches effectively controlled Klebsiella pneumoniae infections in mice. This novel approach reduced bacterial burden in key tissues, showing promise for new antibiotic delivery methods.

Area of Science:

  • Microbiology
  • Pharmacology
  • Biomedical Engineering

Background:

  • Klebsiella pneumoniae is a significant cause of bacterial infections.
  • Effective antibiotic delivery remains a challenge for treating localized infections.
  • Novel drug delivery systems are needed to improve therapeutic outcomes.

Purpose of the Study:

  • To investigate the efficacy of gentamicin dissolving microarray patches for treating Klebsiella pneumoniae infection.
  • To evaluate the in vivo delivery of gentamicin using microarray patch technology.
  • To establish a proof of concept for this novel antimicrobial delivery system.

Main Methods:

  • A murine model of Klebsiella pneumoniae infection was utilized.
  • Gentamicin was administered via dissolving microarray patches applied to murine ears.
  • Bacterial burden was assessed in nasal-associated lymphoid tissue and lungs.

Main Results:

  • Microarray patch application of gentamicin significantly controlled Klebsiella pneumoniae infection.
  • Treated mice exhibited reduced bacterial burden in the nasal-associated lymphoid tissue and lungs compared to controls.
  • This study provides the first in vivo data on gentamicin delivery via dissolving microarray patches.

Conclusions:

  • Dissolving microarray patches offer a viable method for in vivo delivery of gentamicin.
  • This technology demonstrates potential for controlling bacterial infections like Klebsiella pneumoniae.
  • Further research into microarray patch applications for infectious diseases is warranted.
Keywords:
Klebsiella pneumoniaegentamicinmicroarraytransdermal delivery

More Related Videos

Experimental Model to Evaluate Resolution of Pneumonia09:49

Experimental Model to Evaluate Resolution of Pneumonia

Published on: February 17, 2023

1.9K
A Mouse Model for the Transition of Streptococcus pneumoniae from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness12:21

A Mouse Model for the Transition of Streptococcus pneumoniae from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness

Published on: September 28, 2022

3.1K

Related Concept Videos

Pneumonia I: Introduction01:30

Pneumonia I: Introduction

Pneumonia is an acute respiratory infection that targets the lungs, specifically the alveoli. These tiny air sacs, essential for oxygen exchange, become engorged with pus and fluid, severely hindering breathing, decreasing oxygen absorption, and causing significant pain and discomfort during respiration.
Risk Factors
Various factors influence the likelihood of developing pneumonia. Age plays a crucial role, with infants, children under two, and individuals over 65 at increased risk due to their...
852
Pneumonia II: Pathophysiology01:29

Pneumonia II: Pathophysiology

The pathophysiology of pneumonia involves the following steps:
2.8K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
8.0K
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
21.1K
Pneumonia IV: Management01:28

Pneumonia IV: Management

The treatment of pneumonia varies based on its severity and the causative pathogen. Here is a structured approach to managing pneumonia, integrating pharmaceutical and supportive care strategies.
Bacterial Pneumonia Treatment
For bacterial pneumonia, antibiotics serve as the cornerstone of therapy. Initial treatment often begins with empirical antibiotics, tailored to the anticipated causative organism and adjusted based on culture results. Key antibiotic choices include:
803
Pneumonia V: Nursing management and Prevention01:30

Pneumonia V: Nursing management and Prevention

Nursing management of pneumonia involves promoting airway patency, facilitating rest and conserving energy, encouraging fluid intake, maintaining nutrition, and educating patients.
The nurse must practice strict medical asepsis and adhere to infection control guidelines to minimize healthcare-associated infections.
Enhance airway patency
Position the patient correctly to facilitate drainage of the affected lung segments. Manual or mechanical percussion and vibration can also be employed....
3.5K