Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

18.4K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
18.4K
Negative Regulator Molecules01:23

Negative Regulator Molecules

38.5K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.5K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

9.2K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
9.2K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

10.5K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.5K
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

1.2K
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
1.2K
Adrenergic Receptors: β Subtype01:26

Adrenergic Receptors: β Subtype

3.7K
β-adrenoceptors have varied sensitivities towards adrenaline, noradrenaline, and isoprenaline. The order of agonist potency is as follows:
Isoprenaline > Adrenaline > Noradrenaline
Neurotransmitter binding to these receptors causes activation of adenylyl cyclase resulting in increased concentrations of cAMP and modulation of calcium ion channels within the cell. They are further classified into β1, β2, and β3 subtypes.
β1-adrenoceptors: β1-adrenoceptors...
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The phyllosphere as a potential microbial habitat sharing taxa with the Jiang-flavor Baijiu fermentation system: A preliminary study.

Food research international (Ottawa, Ont.)·2026
Same author

Pulmonary surfactant camouflage lipid nanoparticles for efficient mRNA lung delivery.

Signal transduction and targeted therapy·2026
Same author

Molecular rotation and large polarization in charge-transfer ferroelectric cocrystals.

Nature chemistry·2026
Same author

BA-UNet: A Boundary Augmented Segmentation Network for Cervical Cancer Radiotherapy.

Journal of imaging informatics in medicine·2026
Same author

Harnessing confinement effect and interpretable machine learning to predict alkane diffusion in zeolite catalysts.

Nature communications·2026
Same author

Multi-layer causal knowledge network for product quality problem analysis using third data.

Scientific reports·2026
Same journal

Soluble HER2 promotes chronic antigen-mediated CD8⁺ T-cell dysfunction and limits immunotherapy response in HER2-low triple-negative breast cancer.

Cell death & disease·2026
Same journal

Correction: Tumor suppressor function of SHMT in a Drosophila Ras<sup>V12</sup>Dlg<sup>RNAi</sup> model: DNA damage and synergistic gene-nutrient interaction with PLP.

Cell death & disease·2026
Same journal

Correction: The EHMT2-MBLAC2 axis suppresses ribosomal DNA transcription in response to nucleolar DNA damage.

Cell death & disease·2026
Same journal

Phase separation drives NLRP3 inflammasome activation independently of Golgi localization.

Cell death & disease·2026
Same journal

mTORC1 suppression by Trp53 mutation drives resistance to immune checkpoint blockade.

Cell death & disease·2026
Same journal

Disrupting Treg lineage stability elicits RORgT-mediated plasticity and enhances anti-tumor immunity.

Cell death & disease·2026
See all related articles

Related Experiment Video

Updated: Jan 28, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.7K

p53 β-hydroxybutyrylation attenuates p53 activity.

Kun Liu1, Fangzhou Li1, Qianqian Sun1

  • 1Department of Biochemistry and Molecular Biology, Beijing Key Laboratory of Protein Posttranslational Modifications and Cell Function, Peking University Health Science Center, 38 Xueyuan Road, 100191, Beijing, China.

Cell Death & Disease
|March 13, 2019
PubMed
Summary
This summary is machine-generated.

Beta-hydroxybutyrate (BHB) induces a new modification on the tumor suppressor p53, called p53 kbhb. This modification, catalyzed by CBP, impacts p53

More Related Videos

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.0K
Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice
17:59

Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice

Published on: April 3, 2011

12.7K

Related Experiment Videos

Last Updated: Jan 28, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.7K
Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.0K
Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice
17:59

Derivation of Thymic Lymphoma T-cell Lines from Atm-/- and p53-/- Mice

Published on: April 3, 2011

12.7K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • p53 is a critical tumor suppressor protein.
  • Posttranslational modifications regulate p53 activity.
  • Beta-hydroxybutyrylation (Kbhb) is a newly identified histone modification.

Purpose of the Study:

  • To investigate if p53 is subject to beta-hydroxybutyrylation (Kbhb).
  • To determine the functional consequences of p53 Kbhb on p53 activity and downstream gene expression.
  • To explore the role of p53 Kbhb in cancer and its potential as a therapeutic target.

Main Methods:

  • Western blotting to detect p53 modification.
  • Cell culture experiments with BHB treatment.
  • Analysis of p53 downstream gene expression (p21, PUMA).
  • In vivo studies using fasted mice and gamma-irradiation.

Main Results:

  • p53 is modified by Kbhb at specific lysine residues (120, 319, 370).
  • p53 Kbhb levels increase upon BHB treatment and during fasting in vivo.
  • CBP was identified as the enzyme catalyzing p53 Kbhb.
  • p53 Kbhb reduces p53 acetylation, leading to decreased p21 and PUMA expression.
  • Reduced cell growth arrest and apoptosis were observed under p53-activating conditions.

Conclusions:

  • BHB-mediated p53 Kbhb is a novel regulatory mechanism for p53 activity.
  • This modification links ketone bodies to tumor biology.
  • p53 Kbhb represents a potential therapeutic target for cancer treatment.