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Updated: May 5, 2026

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo
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The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

Published on: April 25, 2016

33.6K

c-fos expression is neither sufficient nor obligatory for differentiation of monomyelocytes to macrophages

R L Mitchell, C Henning-Chubb, E Huberman

    Cell
    |May 23, 1986
    PubMed

    Insights

    The c-fos gene

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Gene Expression Regulation

    Background:

    • The c-fos gene is rapidly and transiently expressed during the differentiation of human leukemia cells into macrophages.
    • Understanding the regulation of c-fos expression is crucial for comprehending cellular differentiation processes.

    Purpose of the Study:

    • To investigate the regulatory mechanisms controlling c-fos gene expression during macrophage differentiation.
    • To determine the role of c-fos expression in the differentiation of U-937 and HL-60 leukemia cells.

    Main Methods:

    • Analysis of c-fos mRNA levels and stability in U-937 and HL-60 cells.
    • Induction of differentiation using various stimuli (serum, diacylglycerol, TPA).
    • Assessment of cell differentiation to macrophages.

    Main Results:

    • c-fos expression is primarily controlled at the transcriptional level.
    • c-fos mRNA exhibits a short half-life (less than 30 minutes).
    • Superinduction of c-fos by cycloheximide is due to mRNA stabilization.
    • High c-fos expression does not always lead to macrophage differentiation.
    • Macrophage differentiation can occur without detectable c-fos expression in TPA-resistant HL-60 variants.

    Conclusions:

    • c-fos gene expression regulation is complex and occurs mainly at the transcriptional level.
    • While c-fos is transiently expressed during differentiation, its role is not essential for macrophage differentiation in all contexts.
    • mRNA stability plays a significant role in the observed 'superinduction' of c-fos.

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