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Updated: Jan 27, 2026

Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling
Published on: August 23, 2024
Addressing the Chemistry of Germacrene A by Isotope Labeling Experiments
Jan Rinkel1, Jeroen S Dickschat1
1Kekulé-Institute for Organic Chemistry and Biochemistry , University of Bonn , Gerhard-Domagk-Str. 1 , 53121 Bonn , Germany.
Abstract:
Despite the central role of germacrene A in sesquiterpene biosynthesis and its widespread occurrence in nature, its complete NMR characterization is still pending. This problem was solved through enzymatic preparation of germacrene A isotopomers that allowed for a full signal assignment to all three conformers. The obtained materials gave insights into the stereochemical course of the Cope rearrangement to β-elemene and uncovered the Cope rearrangement as a new EI-MS fragmentation reaction.
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