Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spatial and temporal pattern of hsp26 expression during normal development.

R L Glaser, M F Wolfner, J T Lis

    The EMBO Journal
    |April 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Correlated Gene Copy Number Changes in a Seminal Fluid Protein Network in <i>Drosophila</i>.

    bioRxiv : the preprint server for biology·2025
    Same author

    Corrigendum. Heritable Variation in Courtship Patterns in Drosophila melanogaster.

    G3 (Bethesda, Md.)·2015
    Same author

    The consequences of genetic variation in sex peptide expression levels for egg laying and retention in females.

    Heredity·2012
    Same author

    Improvement in scalp hair growth in androgen-deficient women treated with testosterone: a questionnaire study.

    The British journal of dermatology·2011
    Same author

    Drosophila heat shock system as a general model to investigate transcriptional regulation.

    Cold Spring Harbor symposia on quantitative biology·2011
    Same author

    Local DNA underreplication correlates with accumulation of phosphorylated H2Av in the Drosophila melanogaster polytene chromosomes.

    Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology·2008
    Same journal

    Scalable phosphotyrosine enrichment with SH2 superbinder enables deep profiling of EGF responses.

    The EMBO journal·2026
    Same journal

    Essential nucleus-apical pole linkage maintains division fidelity during Plasmodium progeny formation.

    The EMBO journal·2026
    Same journal

    From cell atlases to mechanisms: bridging scRNA-seq discovery with in vivo genetics.

    The EMBO journal·2026
    Same journal

    Mitochondrial calcium regulates lipid metabolism by modulating tethering of mitochondria to lipid droplets.

    The EMBO journal·2026
    Same journal

    Chromosome condensation mechanically primes the nucleus for mitosis.

    The EMBO journal·2026
    Same journal

    NDR kinase SAX-1 controls dendrite branch-specific elimination during neuronal remodeling in C. elegans.

    The EMBO journal·2026
    See all related articles

    Analyzing heat shock protein 26 (hsp26) gene expression in Drosophila revealed tissue-specific developmental patterns. Deletions in regulatory regions altered expression, highlighting the importance of upstream sequences and chromosomal position.

    Area of Science:

    • Developmental Biology
    • Genetics
    • Molecular Biology

    Background:

    • Understanding gene regulation is crucial for developmental biology.
    • Heat shock proteins (HSPs) play vital roles in cellular stress responses and development.
    • The hsp26 gene in Drosophila melanogaster serves as a model for studying gene expression patterns.

    Purpose of the Study:

    • To analyze the tissue-specific developmental expression patterns of the hsp26 gene in Drosophila.
    • To investigate the regulatory sequences controlling hsp26 expression using lacZ fusion genes.
    • To compare the expression patterns of fusion genes with endogenous hsp26 RNA distribution.

    Main Methods:

    • Germline transformation was used to insert hsp26-lacZ fusion genes into Drosophila melanogaster.

    Related Experiment Videos

  • Histochemical assays for beta-galactosidase activity were performed on whole animals.
  • In situ hybridization with RNA probes was used to determine endogenous hsp26 RNA distribution in tissue sections.

    • Main Results:

    • hsp26 is expressed in multiple tissues during development, including spermatocytes, nurse cells, and neurocytes.
    • A shorter fusion gene construct (278 bp upstream) retained spermatocyte expression but lost nurse cell expression.
    • Chromosomal position effects were observed, leading to novel expression patterns in some transformant lines.

    Conclusions:

    • The hsp26 gene exhibits complex, tissue-specific developmental regulation.
    • Upstream regulatory sequences and chromosomal integration sites significantly influence hsp26 expression patterns.
    • The beta-galactosidase assay provides a valuable tool for studying complex gene regulation in vivo.