Cyanobacteria as regulators of methylmercury production in periphyton

Biotic mercury (Hg) methylation appears to depend on factors such as microbial activity and the concentration and bioavailability of Hg²⁺ to the Hg-methylating organisms. Recently, the presence of cyanobacteria has been linked with high methylmercury (MeHg) concentrations. The aim of this work was to test MeHg production in microcosms, in relation to the amount of periphytic cyanobacteria, dissolved organic matter (DOM) and phosphorus concentrations, as well as periphytic primary production rates. Water and periphyton samples were collected for cultivation and isolation of cyanobacteria from the Guaporé River floodplain, Brazil. We cultivated the periphyton in microcosms with different concentrations of cyanobacteria, total phosphorus and DOM. The highest net MeHg production (6.8 to 24.6% of added Hg d^-1) occurred in the microcosm with added cyanobacteria, followed by microcosms with added phosphorus (6.1 to 11.4%) and added DOM (6.4 to 9.1%). Positive correlations were found between MeHg production, addition of cyanobacteria, phosphorus and DOM and periphytic primary productivity. Our results bring the first direct experimental evidence of the relevance of cyanobacteria and primary production as regulators of MeHg production in periphyton. These findings have numerous implications for the management of natural and engineered wetlands.