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Long Non-coding RNA PVT1 Competitively Binds MicroRNA-424-5p to Regulate CARM1 in Radiosensitivity of Non-Small-Cell

Dong Wang1, Yi Hu2

  • 1Department of Medical Oncology, Chinese PLA General Hospital, Beijing 100853, China; Department of Oncology, Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao 028000, China.

Molecular Therapy. Nucleic Acids
|March 13, 2019
PubMed

Insights

Long non-coding RNA PVT1 promotes non-small-cell lung cancer (NSCLC) radiosensitivity. Silencing PVT1 or increasing microRNA-424-5p enhances NSCLC radiosensitivity by targeting the PVT1/CARM1 pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Dysregulated long non-coding RNAs (lncRNAs) are implicated in cancer development.
  • Non-small-cell lung cancer (NSCLC) radiosensitivity is a critical factor in treatment efficacy.

Purpose of the Study:

  • To investigate the role of lncRNA PVT1 in NSCLC radiosensitivity.
  • To elucidate the molecular mechanism involving the microRNA (miR)-424-5p/lncRNA PVT1/CARM1 signaling pathway.

Main Methods:

  • Bioinformatic analysis to identify differentially expressed lncRNAs and co-expressed genes.
  • Cell culture and transfection with mimics, inhibitors, and siRNAs targeting PVT1 and miR-424-5p.
  • RNA immunoprecipitation (RIP) assay to confirm PVT1-CARM1 interaction.
  • Assessment of cell proliferation, migration, invasion, cell cycle, and apoptosis.

Main Results:

  • lncRNA PVT1 was significantly upregulated in NSCLC and associated with radiosensitivity.
  • PVT1 and CARM1 were found to interact with miR-424-5p.
  • Silencing PVT1 or overexpressing miR-424-5p inhibited NSCLC cell proliferation, migration, and invasion while promoting apoptosis.

Conclusions:

  • lncRNA PVT1 promotes NSCLC radiosensitivity through the miR-424-5p/lncRNA PVT1/CARM1 pathway.
  • Targeting PVT1 or enhancing miR-424-5p expression can improve NSCLC radiosensitivity.
Keywords:
co-activator-associated arginine methyltransferase 1long non-coding plasmacytoma variant translocation 1microRNA-424-5pnon-small-cell lung cancerradiosensitivity

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