Larvicidal activity of selected essential oil in synergized combinations against Aedes aegypti

This study was conducted to attain an alternate plant essential oil (EO) based mosquito larvicide against the dengue vector Aedes aegypti. Here three plants were selected based on their local availability, safety and insecticidal properties. EOs were extracted from Syzygium aromaticum (Myrtaceae) flower buds, fruits of Illicium verum (Schisandraceae) and Trachyspermum ammi (Apiaceae) by hydro-distillation and tested against Ae. aegypti larvae individually and in combinations to find synergistic interactions. Chemical constituent analysis of EOs was done by GC/MS/MS analysis and the main constituents in T. ammi were thymol (93.58%) and terpinen-4-ol (2.16%), in S. aromaticum eugenol 56.32% and caryophyllene 17.91% and in I. verum trans-anethole 53.05% and p-anisaldehyde 12.47%. The EOs from T. ammi, I. verum and S. aromaticum and their top components thymol, trans-anethole and eugenol exhibited larvicidal activity with LC₅₀ values 39.48, 41.30, 66.90, 59.76, 50.19 and 60.89 mgL^-1 respectively against Ae. aegypti larvae. The values for the co-toxicity factors for the binary combinations of the EOs were > 20 showing synergistic interactions among the binary mixtures. The respective LC₅₀ values for the 1:1 binary combinations (S. aromaticum + I. verum), (S. aromaticum + T. ammi) and (I. verum + T. ammi) were 49.07, 48.54 and 27.67 mgL^-1. β-cyclodextrin inclusion complex made with I. verum + T. ammi combination showed an LC₅₀ value of 23.93 mgL^-1. On the whole the outcome of this study draw attention to the capability of synergistic EO combinations to emerge as a safe and environment friendly effective larvicide to control Aedes mosquitoes.