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Transmitting Plant Viruses Using Whiteflies
Published on: November 8, 2013
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Partial Characterization of Two Whitefly-Transmitted Geminiviruses Infecting Tomatoes in Venezuela
P Guzman1, C R Arredondo2, D Emmatty2
1Department of Plant Pathology, University of California, Davis 95616.
Plant Disease
|March 14, 2019
Summary
Two distinct geminiviruses infect tomatoes in Venezuela, causing significant yield losses. One identified virus is closely related to potato yellow mosaic virus (PYMV), while another may be an undescribed species.
Area of Science:
- Plant Pathology
- Virology
- Molecular Biology
Background:
- Whitefly-transmitted geminiviruses cause substantial tomato yield losses in Venezuela.
- Tomato yellow leaf curl virus (TYLCV) is a known pathogen, but other geminiviruses may also be present.
Purpose of the Study:
- To identify and characterize geminiviruses infecting tomatoes in Venezuela.
- To determine the genetic relationship of these viruses to known geminiviruses.
Main Methods:
- Squash blot hybridization and PCR with degenerate primers were used to detect and amplify geminivirus DNA.
- DNA-A fragments from infected tomato and weed samples were sequenced.
- Sequence analysis was performed to determine viral identity and relationships.
Main Results:
- Geminivirus DNA was detected in 19 of 20 tomato samples and one weed sample.
- Sequencing revealed at least two distinct bipartite geminiviruses.
- Three isolates were identified as strains of potato yellow mosaic virus (PYMV), and one isolate may represent a new geminivirus species.
Conclusions:
- At least two distinct bipartite Western Hemisphere whitefly-transmitted geminiviruses are associated with tomato diseases in Venezuela.
- One of these viruses, identified in sample 3R, may be an undescribed geminivirus.
- The weed sample did not host the identified tomato-infecting geminiviruses.
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