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First Report of Grapevine Virus A in Spain
I Zabalgogeazcoa1, C de Blas1, C Cabaleiro2
1Biología Molecular y Virología Vegetal, CIT-INIA, Autopista A-6 Km 7.5, 28040 Madrid.
Plant Disease
|March 14, 2019
Summary
Grapevine trichovirus A (GVA) was detected in Spanish grapevines exhibiting leafroll symptoms using immunocapture-RT-PCR. This study marks the first identification of GVA in Spain, confirming its presence in European viticulture.
Area of Science:
- Plant Virology
- Molecular Diagnostics
- Agricultural Science
Background:
- Grapevine leafroll disease is associated with phloem-limited viruses.
- Grapevine trichovirus A (GVA) is a known pathogen in grapevines globally.
- Previous GVA detection was limited to Mediterranean countries outside Spain.
Purpose of the Study:
- To detect and confirm the presence of Grapevine trichovirus A (GVA) in Spanish grapevines.
- To investigate GVA prevalence in Vitis vinifera cultivars with leafroll symptoms.
- To establish the first report of GVA detection in Spain.
Main Methods:
- Field survey of diseased grapevines in Pontevedra, Spain (1993).
- Immunocapture-reverse transcription-polymerase chain reaction (RT-PCR) with GVA-specific primers.
- Northern blot hybridization using a 32P-labeled GVA PCR product probe for verification.
Main Results:
- GVA was detected in symptomatic Vitis vinifera grapevines using RT-PCR.
- Specific 430 bp bands were amplified from petiole and stem bark extracts.
- Northern blot analysis confirmed GVA presence in 2 out of 10 analyzed samples.
- The GVA probe demonstrated high specificity, not cross-reacting with other viruses or healthy plant tissues.
Conclusions:
- This study provides the first evidence of Grapevine trichovirus A (GVA) detection in Spain.
- GVA is confirmed to be present in Spanish vineyards, contributing to leafroll symptoms.
- The findings expand the known geographical distribution of GVA within Europe.
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