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Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
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The Notch signal mediates macrophage polarization by regulating miR-125a/miR-99b expression

Qian Li1, Xia He1, Qiao Yu2

  • 1a Department of Radiation Oncology , Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University , Nanjing , PR China.

Artificial Cells, Nanomedicine, and Biotechnology
|March 14, 2019
PubMed

Insights

The Notch signaling pathway promotes macrophage polarization to the M1 phenotype by increasing miR-125a/miR-99b expression. This pathway influences key markers and enhances apoptosis in M1 macrophages.

Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • Macrophage polarization is crucial in immune responses.
  • The Notch signaling pathway plays a role in various cellular processes.

Purpose of the Study:

  • To investigate the role of Notch signaling in macrophage polarization.
  • To elucidate the molecular mechanisms linking Notch signaling to M1/M2 polarization.

Main Methods:

  • Utilized Lyz2 cre and RBP-J flox mice for Notch signal knockout in macrophages.
  • Isolated and polarized bone marrow-derived macrophages (BMDMs).
  • Quantified polarization marker expression using qPCR and measured nitric oxide (NO) release.

Main Results:

  • Notch knockout decreased M1 markers and increased M2 markers.
  • miR-125a/miR-99b expression was upregulated in M1 polarization and downregulated in M2.
  • Overexpression of miR-125a promoted M1 markers, M1 macrophage-induced apoptosis, and NO release, while inhibiting M2 markers.

Conclusions:

  • The Notch signaling pathway drives macrophage polarization towards the M1 phenotype.
  • Upregulation of miR-125a/miR-99b is a key mechanism by which Notch signaling promotes M1 polarization.
  • Modulating Notch signaling and miR-125a/miR-99b offers potential therapeutic strategies.
Keywords:
Notch signalmacrophagemiR-125amiR-99b

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