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Related Experiment Video

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Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant

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Published on: September 7, 2016

Functionalized titanium implant in regulating bacteria and cell response.

Jianfeng Jin¹, Dongdong Fei¹, Yumei Zhang²

¹State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, Xi'an, People's Republic of China, yznmbk@fmmu.edu.cn.

International Journal of Nanomedicine

|March 14, 2019

Summary

This study investigates the Ti-GO-Ag nanocomposite for biomedical implants. The research details its antibacterial mechanisms and biocompatibility, offering insights into its dual functionality for dental and orthopedic applications.

Keywords:

Ti-GO-Ag nanocomposite antibacterial mechanism cell behavior functionalized titanium implant material characterization surface topography

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Area of Science:

Biomaterials Science
Nanotechnology
Materials Engineering

Background:

Biological complications in dental and orthopedic implants are a significant concern.
Titanium (Ti), graphene oxide (GO), and silver (Ag) possess desirable properties for biomedical implants.
The combined antibacterial mechanisms of Ti-GO-Ag nanocomposites remain unclear.

Purpose of the Study:

To investigate the material characterization, antibacterial properties, and cell behavior of Ti-GO-Ag.
To elucidate the antibacterial mechanisms of Ti-GO-Ag nanocomposites.
To assess the potential of Ti-GO-Ag as a dual-functionalized implant biomaterial.

Main Methods:

Fabrication of Ti-GO-Ag using electroplating and ultraviolet reduction.
Material characterization via AFM, Raman spectroscopy, XPS, nanoindentation, nanoscratch, ICP-MS, and contact angle testing.
Antibacterial property assessment and cell behavior analysis.

Main Results:

Material characterization confirmed the influence of GO concentration and Ag content.
Detailed observation of bacterial interaction with Ti-GO-Ag, including ROS generation, endocytosis, aggregation, perforation, and leakage.
Ti-GO-Ag influenced cell area, length, width, and fluorescence intensity.

Conclusions:

Ti-GO-Ag nanocomposite exhibits dual functionality as an antibacterial and biocompatible implant material.
The study provides a comprehensive understanding of the antibacterial mechanisms and cell interactions.
This research supports the development of advanced biomaterials for improved implant performance.