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Updated: Jan 27, 2026
07:14
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
Published on: May 17, 2021
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A modified method for isolating human quiescent pancreatic stellate cells
Fu-Tao Meng1,2, Mei Huang1,2, Fang-Fang Fan3
1Department of General Surgery, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei, Anhui Province, People's Republic of China, ahslyyhq@sohu.com.
Cancer Management and Research
|March 14, 2019
Summary
Researchers developed a simple, high-yield method to isolate quiescent human pancreatic stellate cells (PSCs), ensuring a reliable source for pancreatic stellate cell studies.
Area of Science:
- Cell Biology
- Gastroenterology
- Tissue Engineering
Background:
- Human pancreatic stellate cells (PSCs) are crucial in pancreatic research.
- A consistent and abundant supply of quiescent PSCs is needed for reliable study outcomes.
Purpose of the Study:
- To establish a straightforward, high-yield protocol for isolating quiescent human pancreatic stellate cells (NPSCs).
- To ensure a stable source of high-purity NPSCs for downstream research applications.
Main Methods:
- Utilized gentleMACS™ tissue processor for efficient single-cell suspension preparation from human pancreatic tissue.
- Employed Percoll density gradient centrifugation for the isolation of quiescent normal PSCs (NPSCs).
- Assessed cell viability via trypan blue staining and NPSC state through autofluorescence and oil red O staining; human activated PSC (APSC) purity was confirmed by immunofluorescence.
Main Results:
- Achieved a yield of approximately (2.75±0.65)×10^6 NPSCs per gram of pancreatic tissue.
- Demonstrated maximum cell viability of 92% and maximum cell purity of 95% for isolated NPSCs.
Conclusions:
- The developed method offers a simple, high-yield, and stable approach for isolating quiescent human pancreatic stellate cells.
- This protocol is recommended for widespread adoption in PSC-related research due to its efficiency and reliability.
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