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Cis-regulatory Sequences

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

Cis-regulatory Sequences

Cis-regulatory Sequences

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

Per-Unit Sequence Models

Per-Unit Sequence Models

An ideal Y-Y transformer, grounded through neutral impedances, displays per-unit sequence networks akin to those of a single-phase ideal transformer when subjected to balanced positive- or negative-sequence currents. These currents do not produce neutral currents, and their associated voltage drops.
Zero-sequence currents, which are identical in magnitude and phase, generate a neutral current, resulting in voltage drops across the neutral impedance and the low-voltage winding. If the...

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Related Experiment Video

Updated: Jan 27, 2026

Detection of Copy Number Alterations Using Single Cell Sequencing

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

Corrigendum: A Single-Cell Sequencing Guide for Immunologists.

Peter See¹, Josephine Lum¹, Jinmiao Chen¹

¹Singapore Immunology Network, Agency for Science, Technology and Research, Singapore, Singapore.

Frontiers in Immunology

|March 14, 2019

Summary

This study corrects a previously published article DOI. The correction ensures accurate citation and referencing for future research in the field.

Keywords:

10X genomics chromium MARS-seq SMART-seq dendritic cells fluidigm C1 immunology single-cell RNA sequencing

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Area of Science:

Immunology
Biomedical Research

Context:

Correction of a previously published article DOI.
Ensuring accurate scientific record-keeping.

Purpose:

To rectify an error in the article's digital object identifier.
To maintain the integrity of scientific literature.

Summary:

The article DOI 10.3389/fimmu.2018.02425 has been corrected.
This ensures proper attribution and accessibility of the research.

Impact:

Improved accuracy in scientific citations.
Facilitates easier retrieval and referencing of the research article.