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The Construction of Full-Length cDNA Library for Otodectes cynotis
Li Hu1, Ya-E Zhao2, Dong-Ling Niu1
1Department of Pathogenic Biology and Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University, No.76 Yanta West Road, Xi'an, 710061, China.
Acta Parasitologica
|March 14, 2019
Summary
A reliable cDNA library for Otodectes cynotis, the cause of mange in pets, was successfully created. This breakthrough enables further research into the mite
Area of Science:
- Veterinary Parasitology
- Molecular Biology
- Genomics
Background:
- Otodectes cynotis causes mange in carnivores worldwide.
- Research on O. cynotis pathogenesis is limited by the absence of a cDNA library.
- This mite can also infest humans.
Purpose of the Study:
- To construct a reliable full-length cDNA library for O. cynotis.
- To facilitate future research on O. cynotis gene function and RNA sequencing.
Main Methods:
- RNA extraction from O. cynotis mites.
- Construction of a full-length cDNA library using SMART technique.
- Sequencing and analysis of positive clones and gene fragments.
Main Results:
- High-quality RNA was extracted from 300 mites.
- The constructed cDNA library met quality standards with a titer of 5.02 × 10^5 PFU/ml.
- Sequenced clones showed high identity to known O. cynotis genes in GenBank.
Conclusions:
- A successful and reliable cDNA library for O. cynotis was established.
- This library provides a foundation for advanced molecular studies of O. cynotis.
- Enables future research on RNA sequencing and functional genomics of the mite.
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