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RecET Direct Cloning of Polysaccharide Gene Cluster from Gram-Negative Bacteria
Zhongrui Ma1, Peng George Wang2
1Department of Chemistry, Georgia State University, Atlanta, GA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 14, 2019
Abstract:
RecET direct cloning enables obtaining a large DNA region from genome. Here we describe its applications in cloning of polysaccharide gene cluster from gram-negative bacteria. Rapid and exact cloning of polysaccharide gene cluster can be achieved by this method.
Keywords:
ElectroporationGenome isolationGram-negative bacteriaLinear cloning vectorPolysaccharide gene clusterRecET direct cloningRestriction enzyme digestion
