Recovery of Natural α-Ionone from Fermentation Broth

Recently, the market value of aromas has constantly been rising. Because the supply from natural feedstock is limited, the biotechnological production has received more interest. Thus far, only a few attempts have been made to produce α-ionone, a valued essential aroma of raspberry, biotechnologically. This study reports a production process for enantiopure (R)-α-ionone from lab scale (2-150 L) with typical titer of 285 mg/L broth to industrial scale (up to 10 000 L) with a titer up to 400 mg/L broth, focusing on the development of a downstream process with a maximized yield at minimized effort. The developed recovery consists of solid-liquid extraction from the biomass at φ = 0.4 g of n-hexane/g of biomass for 90 min at ambient temperature and adsorption from the aqueous supernatant at Φ = 0.5 g of Diaion HP-20/mg of α-ionone, followed by desorption at Ψ = 30 g of n-hexane/g of Diaion HP-20. Altogether, natural α-ionone could be gained in substantial quantity and purity of >95%.