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Updated: Jan 27, 2026

Super-resolution Imaging of Neuronal Dense-core Vesicles

Super-resolution Imaging of Neuronal Dense-core Vesicles

Published on: July 2, 2014

124-Color Super-resolution Imaging by Engineering DNA-PAINT Blinking Kinetics.

Orsolya K Wade^1,2, Johannes B Woehrstein^1,2, Philipp C Nickels^1,2

¹Department of Physics and Center for Nanoscience , Ludwig Maximilian University , 80539 Munich , Germany.

|March 14, 2019

Summary

This study introduces kinetic barcoding for multiplexed super-resolution microscopy, enabling simultaneous detection of hundreds of molecular species. The novel DNA-PAINT approach achieves 124-plex imaging in minutes for biological applications.

Keywords:

DNA nanotechnology DNA-PAINT Super-resolution microscopy barcoding multiplexing

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Area of Science:

Biophysics
Molecular Imaging
Nanotechnology

Background:

Optical super-resolution microscopy offers nanoscale spatial resolution.
Efficient multiplexing for simultaneous detection of numerous molecular targets remains a challenge.

Purpose of the Study:

To develop a novel multiplexing strategy for super-resolution microscopy.
To enable the simultaneous detection of hundreds of molecular species with high resolution.

Main Methods:

Engineering blinking behavior of targets using DNA-PAINT with controlled binding kinetics.
In silico and in vitro validation using DNA origami structures.
Application for multiplexed RNA and protein detection in cellular environments.

Main Results:

Demonstrated applicability of kinetic barcoding for multiplexed imaging in cells.
Achieved 124-plex super-resolution imaging within minutes.
Validated the approach using DNA origami and cellular samples.

Conclusions:

Kinetic barcoding is a viable strategy for highly multiplexed super-resolution microscopy.
This method significantly advances the capability for simultaneous molecular detection.
The technique offers rapid and efficient high-plex imaging for biological research.