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Updated: Jan 27, 2026

Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration
Published on: March 17, 2023
Collaborative Study of Alkaline Phosphatase Activity in Filter Paper Disks Impregnated with Skim Milk: Positive
1U.S. Department of Health and Human Services, Public Health Service, Food and Drug Administration, Division of Microbiology, 1090 Tusculum Avenue, Cincinnati, Ohio 45226.
Abstract:
The rapid colorimetric test was used in a collaborative study to determine alkaline phosphatase activity in filter paper disks impregnated with skim milk then dried and stored for several months at room temperature. Five samples of filter paper disks (0 to 6 μg phenol/disk) in duplicate were sent to six collaborators for analysis. Computations of analytical and analyst errors showed variations of 22.2 to 48.8%. Most of the variations were due to differences among analysts, but some were partly due to differences in the slopes of the calibration curves (a = 0.05 level) they prepared at the time of analysis. Collaborator's performance was evaluated by comparing % correct results that were positive (negative) with the expected results. About 95% of the samples were correctly analyzed.
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